3D differential phase contrast microscopy
3D differential phase contrast microscopy
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DOI：
10.1364/boe.7.003940
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发表时间：
2016-10-01
影响因子：
3.4
通讯作者：
Waller, Laura
中科院分区：
文献类型：
--
作者：
Chen, Michael;Tian, Lei;Waller, Laura
我们演示了使用可编程 LED 阵列源捕获的部分相干强度图像的 3D 相位和吸收恢复。图像是通过四种不同的照明模式通过焦点捕获的。使用一阶玻恩和弱物体近似 (WOA)，导出线性 3D 微分相差 (DPC) 模型。部分相干传递函数将样品的复折射率分布与不同散焦下的强度测量相关联。体积重建是通过基于 FFT 的全局方法实现的，无需中间的 2D 相位检索步骤。由于照明在空间上部分相干，因此重建场的横向分辨率达到相干系统数值孔径的两倍，并提高了轴向分辨率。 (C) 2016年美国光学学会
We demonstrate 3D phase and absorption recovery from partially coherent intensity images captured with a programmable LED array source. Images are captured through-focus with four different illumination patterns. Using first Born and weak object approximations (WOA), a linear 3D differential phase contrast (DPC) model is derived. The partially coherent transfer functions relate the sample's complex refractive index distribution to intensity measurements at varying defocus. Volumetric reconstruction is achieved by a global FFT-based method, without an intermediate 2D phase retrieval step. Because the illumination is spatially partially coherent, the transverse resolution of the reconstructed field achieves twice the NA of coherent systems and improved axial resolution. (C) 2016 Optical Society of America