Human B cell development. II. Subpopulations in the human fetus.
Human B cell development. II. Subpopulations in the human fetus.
复制标题
人类 B 细胞发育。
作者：
M. Bofill;G. Janossy;M. Jánossa;G. Burford;G. Seymour;P. Wernet;E. Kelemen
在人的胎儿发育过程中，B细胞群在不同的组织部位表现出不同的表型。胎儿肝脏和骨髓的前B淋巴细胞和B淋巴细胞表达IgM和B细胞标志物B1 （CD20）和BA-1 （CD24）。这些“早期”细胞对其他一些试剂呈阴性，如抗igd、RFB4 （CD22）、RFB6 （CD21）和RFA-2，这些试剂可识别外周B细胞。这些外周B淋巴细胞在发育中的胎儿是异质的。弥漫性分布的B细胞在最早的淋巴结样本中，16至17周的胎龄，以及16至21周的脾脏，是强烈的IgM+ (IgD+,RFB4+，RFB6+和RFA-2+)，但缺乏T细胞相关标记，如T1 （CD5, 67,000道尔顿相当于小鼠的y-1）和T1 -33。在胎儿淋巴结中，从17周开始，原发性结节在滤泡树突状细胞（FD）周围形成，并且包含几乎纯的B细胞群；B1+,BA1+,RFB4+,RFB6+,RFA-2+，它们同时表达IgM，IgD和T细胞相关抗原T1 （CD5）。这些IgM+、T1+细胞的相当大的亚群也对另一种T细胞相关标记物<s:1> -33呈阳性。在脾脏中，IgM+、IgD+、T1+型B细胞数量较少，出现时间较晚，大约在22周左右。这些细胞最初是弥漫性分布的，当小FD细胞团在妊娠第23周出现时，它们开始聚集在周围。此时，IgM+、T1+B细胞也可从腹膜腔和胸膜腔中冲洗出来。T1+，IgM+B细胞可能代表B慢性淋巴样白血病和中心细胞性淋巴瘤的正常等效细胞，并且似乎是小鼠中Ly-1+，IgM+B细胞的对应物。
In man, during fetal development the B cell populations show distinct phenotypes at different tissue sites. The pre-B and B lymphocytes of the fetal liver and bone marrow express IgM and B cell markers, B1 (CD20) and BA-1 (CD24). These "early" cells are negative with a number of other reagents, anti-IgD, RFB4 (CD22), RFB6 (CD21), and RFA-2, which on the other hand recognize peripheral B cells. These peripheral B lymphocytes in the developing fetus are heterogeneous. The diffusely distributed B cells in the earliest lymph node samples, 16 to 17 wk of gestational age, and from 16 to 21 wk in the spleen, are strongly IgM+ (IgD+,RFB4+,RFB6+, and RFA-2+) but lack T cell-associated markers such as T1 (CD5, p 67,000 dalton equivalent of murine Ly-1) and Tü-33. In fetal lymph nodes, primary nodules develop around the follicular dendritic (FD) cells from 17 wk onward, and contain a virtually pure population of B cells; B1+,BA1+,RFB4+,RFB6+,RFA-2+, which simultaneously express IgM,IgD together with T1 (CD5), a T cell-associated antigen. A sizeable subpopulation of these IgM+,T1+ cells are also positive for Tü-33, another T cell-associated marker. In the spleen, the B cells of the IgM+,IgD+,T1+ type appear in smaller numbers and only relatively late around wk 22. These cells are diffusely distributed at first, and start accumulating around the small FD cell clusters as soon as these emerge about the 23rd gestational wk. At that time, the IgM+,T1+B cells can also be washed out from the peritoneal and pleural cavities. The T1+,IgM+B cells may represent the normal equivalent cells of B chronic lymphoid leukemia and centrocytic lymphoma, and appear to be the counterpart of Ly-1+,IgM+B cells in the mouse.