A comparison of three fission yeast mitochondrial genomes
A comparison of three fission yeast mitochondrial genomes
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DOI：
10.1093/nar/gkg134
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发表时间：
2003-01-15
影响因子：
14.9
通讯作者：
Lang, BF
中科院分区：
文献类型：
--
作者：
Bullerwell, CE;Leigh, J;Lang, BF
裂殖酵母属于裂殖菌目，是子囊菌门中一个潜在的深层分支。虽然大量的分子信息可从粟酒裂殖酵母，一个模式真核生物，很少是可从该组的其他成员。为了更好地表征该真菌谱系中的线粒体基因组进化，将另外两种分裂酵母，裂殖酵母（Schizoschiomycessporus）和裂殖酵母（Schizoschiomycessporivar.）测序。而粟酒裂殖酵母的mtDNA只有19 431 bp，而裂殖酵母的mtDNA只有44 227 bp，而裂殖酵母的mtDNA只有19 431 bp，裂殖酵母的mtDNA只有44 227 bp。超过80 kb。这些mtDNA的大小变化主要是由于非编码区。后两种mtDNA的基因含量与已测序的粟酒裂殖酵母mtDNA的基因含量基本一致，后者编码25种tRNA，即大、小线粒体核糖体RNA（rnl和rns）、线粒体RNaseP的RNA组分（rnpB）、线粒体小亚基核糖体蛋白3（rps 3）、细胞色素氧化酶亚基1、2和3（cox 1、cox 2和cox 3）和ATP合酶亚基6、8和9（atp 6、atp 8和atp 9）。然而，trnI 2（cau）（C修饰为赖氨酸）在链孢酵母mtDNA中缺失，其蛋白质编码基因中相应的ATA密码子也缺失，并且在链孢酵母的mtDNA中未发现rps 3和rnpB。- 是的该菌的线粒体DNA含有5个双发夹元件，这是首次在子囊菌中发现。这项研究提供了进一步的证据，有利于这些元素的流动性，并支持他们在线粒体基因组重排的作用。我们的系统发育分析的结果支持单系的裂殖菌目，但质疑他们的分组内Archiascomycota。
The fission yeasts are members of the fungal order Schizosaccharomycetales, a candidate deep-diverging group within Ascomycota. Although a great deal of molecular information is available from Schizosaccharomyces pombe, a model eukaryote, very little is available from other members of this group. In order to better characterize mitochondrial genome evolution in this fungal lineage, the mitochondrial DNA (mtDNA) of two additional fission yeasts, Schizosaccharomyces octosporus and Schizosaccharomyces japonicus var. japonicus, was sequenced. Whereas the mtDNA of S.pombe is only 19 431 bp, the mtDNA of S.octosporus is 44 227 bp, and that of S.japonicus var. japonicus is over 80 kb. The size variation of these mtDNAs is due largely to non-coding regions. The gene content in the latter two mtDNAs is almost identical to that of the completely sequenced S.pombe mtDNA, which encodes 25 tRNA species, the large and small mitochondrial ribosomal RNAs (rnl and rns), the RNA component of mitochondrial RNaseP (rnpB), mitochondrial small subunit ribosomal protein 3 (rps3), cytochrome oxidase subunits 1, 2 and 3 (cox1, cox2 and cox3) and ATP-synthase subunits 6, 8 and 9 (atp6, atp8 and atp9). However, trnI2(cau) (C modified to lysidine) is absent in the S.octosporus mtDNA, as are corresponding ATA codons in its protein-coding genes, and rps3 and rnpB are not found in the mtDNA of S.japonicus var. japonicus. The mtDNA of S.octosporus contains five double hairpin elements, the first report of these elements in an ascomycete. This study provides further evidence in favor of the mobility of these elements, and supports their role in mitochondrial genome rearrangement. The results of our phylogenetic analysis support the monophyly of the Schizosaccharomycetales, but question their grouping within the Archiascomycota.