Imaging local brain activity of multiple freely moving mice sharing the same environment
Imaging local brain activity of multiple freely moving mice sharing the same environment
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DOI：
10.1038/s41598-019-43897-x
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发表时间：
2019-05-16
影响因子：
4.6
通讯作者：
Nagai, Takeharu
中科院分区：
文献类型：
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作者：
Inagaki, Shigenori;Agetsuma, Masakazu;Nagai, Takeharu
电生理场电位动力学已被广泛用于研究大脑功能和相关的精神疾病。考虑到最近的需求，其适用于自由移动的主题，特别是动物在一个群体和社会相互作用，在这里，我们提出了一种新的方法的基础上，生物发光电压指示器LOTUS-V。使用我们的纤维无记录方法的基础上，LOTUS-V，我们成功地捕捉大脑活动的动态变化在自由移动的小鼠。由于LOTUS-V是比率指标，因此未显著检测到运动和头部角度伪影。利用我们的方法作为无纤维系统，我们进一步成功地同时记录了多个独立运动的小鼠，这些小鼠彼此自由互动。重要的是，这使我们能够发现，初级视觉皮层，视觉处理的中心，在小鼠的互动过程中被激活。这种方法可能进一步促进神经生物学和精神病学的广泛研究。
Electrophysiological field potential dynamics have been widely used to investigate brain functions and related psychiatric disorders. Considering recent demand for its applicability to freely moving subjects, especially for animals in a group and socially interacting with each other, here we propose a new method based on a bioluminescent voltage indicator LOTUS-V. Using our fiber-free recording method based on the LOTUS-V, we succeeded in capturing dynamic change of brain activity in freely moving mice. Because LOTUS-V is the ratiometric indicator, motion and head-angle artifacts were not significantly detected. Taking advantage of our method as a fiber-free system, we further succeeded in simultaneously recording from multiple independently-locomotive mice that were freely interacting with one another. Importantly, this enabled us to find that the primary visual cortex, a center of visual processing, was activated during the interaction of mice. This methodology may further facilitate a wide range of studies in neurobiology and psychiatry.