Visualization of embryonic neural stem cells using Hes promoters in transgenic mice
Visualization of embryonic neural stem cells using Hes promoters in transgenic mice
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DOI：
10.1016/j.mcn.2005.09.006
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发表时间：
2006-01-01
影响因子：
3.5
通讯作者：
McConnell, SK
中科院分区：
文献类型：
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作者：
Ohtsuka, T;Imayoshi, I;McConnell, SK
在中枢神经系统中，神经干细胞在脑室区（VZ）增殖，并以时间和空间调节的方式依次产生神经元和胶质细胞，这表明干细胞在不同的脑区域和不同的发育阶段可能彼此不同。为了标记和纯化神经干细胞以确定是否存在这种差异，我们使用来自Hes基因的启动子（pHes 1或pHes 5）来驱动不稳定的增强型绿色荧光蛋白的表达来产生转基因小鼠。在这些转基因小鼠发育中的大脑中，GFP表达仅限于VZ中的未分化细胞，其可以在克隆培养中不对称地产生Numb阳性神经元子代和GFP阳性祖细胞，表明它们保留了自我更新的能力。我们的研究结果表明，pHes-EGFP转基因小鼠可用于探索神经干细胞在发育过程中的相似性和差异。(C)2005年爱思唯尔公司All rights reserved.
In the central nervous system, neural stem cells proliferate in the ventricular zone (VZ) and sequentially give rise to both neurons and glial cells in a temporally and spatially regulated manner, suggesting that stem cells may differ from one another in different brain regions and at different developmental stages. For the purpose of marking and purifying neural stem cells to ascertain whether such differences exist, we generated transgenic mice using promoters from Hes genes (pHes1 or pHes5) to drive expression of destabilized enhanced green fluorescent protein. In the developing brains of these transgenic mice, GFP expression was restricted to undifferentiated cells in the VZ, which could asymmetrically produce a Numb-positive neuronal daughter and a GFP-positive progenitor cell in clonal culture, indicating that they retain the capacity to self-renew. Our results suggest that pHes-EGFP transgenic mice can be used to explore similarities and differences among neural stem cells during development. (C) 2005 Elsevier Inc. All rights reserved.