The chemokine SDF-1 is a chemoattractant for human CD34+ hematopoietic progenitor cells and provides a new mechanism to explain the mobilization of CD34+ progenitors to peripheral blood.
The chemokine SDF-1 is a chemoattractant for human CD34+ hematopoietic progenitor cells and provides a new mechanism to explain the mobilization of CD34+ progenitors to peripheral blood.
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DOI：
10.1084/jem.185.1.111
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发表时间：
1997-01-06
期刊：
影响因子：
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通讯作者：
Gutierrez-Ramos JC
中科院分区：
文献类型：
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作者：
Aiuti A;Webb IJ;Bleul C;Springer T;Gutierrez-Ramos JC
造血祖细胞在体外和体内向基质细胞产生的趋化因子基质细胞衍生因子-1（SDF-1）的梯度迁移。这是第一个报道的人CD 34+祖细胞的化学引诱物。诱导趋化性的SDF-1浓度也诱导CD 34+细胞胞浆钙离子的短暂升高。SDF-1诱导的趋化性被百日咳毒素抑制，表明其在CD 34+细胞中的信号传导是由与Gi蛋白偶联的7个跨膜受体介导的。迁移至SDF-1的CD 34+细胞包括具有更原始（CD 34 +/CD 38-或CD 34 +/DR-）表型的细胞以及表型定型为红系、淋巴系和髓系的CD 34+细胞，包括功能性BFU-E、CFU-GM和CFU-MIX祖细胞。IL-3在体外增加了CD 34+细胞对SDF-1的趋化性，外周血中的CD 34+祖细胞比骨髓中的CD 34+祖细胞的趋化性低，这表明对SDF-1的反应改变可能与CD 34祖细胞动员有关。
Hematopoietic progenitor cells migrate in vitro and in vivo towards a gradient of the chemotactic factor stromal cell-derived factor-1 (SDF-1) produced by stromal cells. This is the first chemoattractant reported for human CD34+ progenitor cells. Concentrations of SDF-1 that elicit chemotaxis also induce a transient elevation of cytoplasmic calcium in CD34+ cells. SDF-1-induced chemotaxis is inhibited by pertussis toxin, suggesting that its signaling in CD34+ cells is mediated by seven transmembrane receptors coupled to Gi proteins. CD34+ cells migrating to SDF-1 include cells with a more primitive (CD34+/CD38− or CD34+/DR−) phenotype as well as CD34+ cells phenotypically committed to the erythroid, lymphoid and myeloid lineages, including functional BFU-E, CFU-GM, and CFU-MIX progenitors. Chemotaxis of CD34+ cells in response to SDF-1 is increased by IL-3 in vitro and is lower in CD34+ progenitors from peripheral blood than in CD34+ progenitors from bone marrow, suggesting that an altered response to SDF-1 may be associated with CD34 progenitor mobilization.