N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide(W-7), a calmodulin antagonist, also inhibits phospholipid-sensitive calcium-dependent protein kinase.
N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide(W-7), a calmodulin antagonist, also inhibits phospholipid-sensitive calcium-dependent protein kinase.
复制标题
N-(6-氨基己基)-5-氯-1-萘磺酰胺(W-7) 是一种钙调蛋白拮抗剂，也能抑制磷脂敏感的钙依赖性蛋白激酶。
DOI：
10.1016/0304-4165(83)90284-2
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Kuo,JF
中科院分区：
文献类型：
--
作者：
Schatzman,RC;Raynor,RL;Kuo,JF
N-（6-Aminohexyl）-5-chloro-1-naphthalensulfonamide（W-7）是一种钙调素拮抗剂，它能抑制磷脂敏感的Ca ~（2+）依赖性蛋白激酶，也能抑制环GMP和环AMP依赖性蛋白激酶。对同种脾磷脂敏感性Ca ~（2+）依赖性蛋白激酶的抑制动力学研究表明，W-7对该酶的抑制作用与磷脂（Ki= 60μM）竞争，而N-（6-氨基己基）-1-萘磺酰胺（W-5）的抑制作用略弱于W-7。研究结果表明，W-6能够抑制多种蛋白激酶，除了那些需要钙调素以前报道。
N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), commonly regared as a calmodulin antagonist, inhibted phospholipid-sensitive Ca2+-dependent protein kinase and to a lesser extent cyclic GMP- and cyclic AMP-dependent protein kinases. Kinetic studies of the inhibition of the homogenous spleen phospholipid-sensitive Ca2+-dependent protein kinase indicated that W-7 inhibited the enzyme activity competitively with respect to phospholipid (Ki= 60μM).N-(6-Aminohexyl)-1-naphthalenesulfonamide (W-5) was found to be musch less potent than W-7. The findings indicate that W-6 was able to inhibit a variety of protein kinases, in addition to those requiring calmodulin previously reported.