Characteristics of Responses of Golgi Cells and Mossy Fibers to Eye Saccades and Saccadic Adaptation Recorded from the Posterior Vermis of the Cerebellum
Characteristics of Responses of Golgi Cells and Mossy Fibers to Eye Saccades and Saccadic Adaptation Recorded from the Posterior Vermis of the Cerebellum
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DOI：
10.1523/jneurosci.4791-08.2009
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发表时间：
2009-01-07
影响因子：
5.3
通讯作者：
Thier, Peter
中科院分区：
文献类型：
--
作者：
Prsa, Mario;Dash, Suryadeep;Thier, Peter
小脑颗粒层的解剖结构表明高尔基细胞（GC）在向浦肯野细胞传递苔藓纤维（MF）传入的通路中具有重要功能。基于这样的解剖观察，早期的建议归因于“增益控制”的GC，最近的调查，其中断言，GC，而不是有助于振荡机制的功能有争议的作用。然而，基于小脑依赖行为的研究支持/驳回增益控制命题的确凿的生理证据一直缺乏。我们解决了这个interneuron的可能功能，通过记录大量的MF和GC在扫视眼球运动过程中从猴小脑的同一皮层区域，即眼蚓部（OMV）的活动。我们的细胞鉴定符合先前建立的标准，主要是与生理参数和细胞形态相关的细胞标记研究。MF和GC的响应模式高度异质。MF放电与眼扫视指标和时间呈线性相关，显示方向偏好和精确的方向调谐。与此相反，GC放电没有强烈的相关性与指标或方向的运动。他们的放电特性也不受运动学习在扫视适应。因此，OMV通过MF通路从不同的眼动区域接收大量关于眼球运动的信息，这在GC中没有反映出来。GC的非特异性对颗粒层中神经元加工的复杂性具有重要意义，这清楚地否定了它们参与增益控制，而是表明这些中间神经元具有更隐蔽的作用。
The anatomical organization of the granular layer of the cerebellum suggests an important function for Golgi cells (GC) in the pathway conveying mossy fiber (MF) afferents to Purkinje cells. Based on such anatomic observations, early proposals have attributed a role in "gain control" for GCs, a function disputed by recent investigations, which assert that GCs instead contribute to oscillatory mechanisms. However, conclusive physiological evidence based on studies of cerebellum-dependent behavior supporting/dismissing the gain control proposition has been lacking as of yet. We addressed the possible function of this interneuron by recording the activity of a large number of both MFs and GCs during saccadic eye movements from the same cortical area of the monkey cerebellum, namely the oculomotor vermis (OMV). Our cellular identification conformed to previously established criteria, mainly to juxtacellular labeling studies correlating physiological parameters with cell morphology. Response patterns of both MFs and GCs were highly heterogeneous. MF discharges correlated linearly with eye saccade metrics and timing, showing directional preference and precise direction tuning. In contrast, GC discharges did not correlate strongly with the metrics or direction of movement. Their discharge properties were also unaffected by motor learning during saccadic adaptation. The OMV therefore receives a barrage of information about eye movements from different oculomotor areas over the MF pathway, which is not reflected in GCs. The unspecificity of GCs has important implications for the intricacies of neuronal processing in the granular layer, clearly discrediting their involvement in gain control and instead suggesting a more secluded role for these interneurons.