IDENTIFICATION OF ESCHERICHIA-COLI DNA HELICASE-I AS THE TRAI GENE-PRODUCT OF THE F-SEX FACTOR
IDENTIFICATION OF ESCHERICHIA-COLI DNA HELICASE-I AS THE TRAI GENE-PRODUCT OF THE F-SEX FACTOR
复制标题
DOI：
10.1073/pnas.80.15.4659
复制
发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
KLINKERT, MQ
中科院分区：
文献类型：
--
作者：
ABDELMONEM, M;TAUCHERSCHOLZ, G;KLINKERT, MQ
能从大肠杆菌F+菌株中分离到具有活性的DNA解旋酶I(相对分子质量为180,000)，但不能从F-菌株中分离到。通过接合将F性因子转移到F-菌株，可以从接合菌株中纯化酶。由此得出结论，解旋酶I由F因子的一部分编码。用携带F因子转移区不同DNA片段的重组质粒同样获得的结果表明，DNA解旋酶I与F因子转移基因之一的TraI的产物是相同的。
Active DNA helicase I (MW 180,000) can be isolated from E. coli F+ strains but not F- strains. The transfer of the F sex factor to F- strains by conjugation permits the purification of the enzyme from the transconjugant strains. It is concluded from this that helicase I is coded for by a portion of the F factor. Results also obtained buy using recombinant plasmids carrying different DNA fragments of the F factor transfer region suggests that DNA helicase I is identical to the product of traI, one of the transfer genes of the F factor.