Triplet repeats form secondary structures that escape DNA repair in yeast
Triplet repeats form secondary structures that escape DNA repair in yeast
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DOI：
10.1073/pnas.96.4.1504
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发表时间：
1999-02-16
影响因子：
11.1
通讯作者：
Petes, TD
中科院分区：
文献类型：
--
作者：
Moore, H;Greenwell, PW;Petes, TD
几种人类神经退行性疾病由CTG/CAG或CGG/CCG三联体重复的扩增引起。单链CNG重复序列在体外形成发夹样结构的发现导致了DNA二级结构形成是扩增机制的重要组成部分的假设。我们发现，含有10 CTG/CAG或CGG/CCG重复的单链DNA环在酿酒酵母减数分裂重组过程中修复效率低下。回文序列和非回文序列的DNA环修复的比较表明，这种低效的修复反映了这些序列在体内形成发夹结构的能力。
Several human neurodegenerative diseases result from expansion of CTG/CAG or CGG/CCG triplet repeats. The finding that single-stranded CNG repeats form hairpin-like structures in vitro has led to the hypothesis that DNA secondary structure formation is an important component of the expansion mechanism. We show that single-stranded DNA loops containing 10 CTG/CAG or CGG/CCG repeats are inefficiently repaired during meiotic recombination in Saccharomyces cerevisiae. Comparisons of the repair of DNA loops with palindromic and nonpalindromic sequences suggest that this inefficient repair reflects the ability of these sequences to form hairpin structures in vivo.