Measurement of Ex Vivo Porcine Lens Shape During Simulated Accommodation, Before and After fs-Laser Treatment.
Measurement of Ex Vivo Porcine Lens Shape During Simulated Accommodation, Before and After fs-Laser Treatment.
复制标题
飞秒激光治疗前后模拟调节过程中离体猪晶状体形状的测量
DOI：
10.1167/iovs.14-16185
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发表时间：
2015
影响因子：
4.4
通讯作者：
Ripken T
中科院分区：
文献类型：
--
作者：
Hahn J;Fromm M;AL Halabi F;Besdo S;Lubatschowski H;Krüger A;Ripken T
目的：根据亥姆霍兹的说法，调节是基于晶状体的柔韧性，晶状体的柔韧性随着年龄的增长而降低，从而导致老视。透镜的柔韧性随着年龄的增长而降低，从而导致老视。利用飞秒（fs）晶状体切开术治疗，可以恢复老花眼晶状体的灵活性。可以使用基于实验数据的有限元方法系统地评估处理的效率。本研究的目的是量化的形状变化的离体晶状体在不同的适应状态，根据FS-晶状体切开术treatment.Methods：5个镜头与睫状体切除离体猪眼（年龄：约6个月，确切年龄未知）被拉伸的调节装置之前和之后的激光治疗。根据调节状态，使用光学相干断层扫描（OCT）测量根据透镜厚度、直径和前后曲率重建的透镜形状。对完整的透镜形状进行参数化，并将每个测量参数与对照组的结果进行比较（n= 5，年龄：约6个月，确切年龄未知）。治疗后，厚度（+140%）、直径（+54%）和前曲率半径（+57%）参数的振幅显著增加对照组与治疗前比较差异无显著性（P> 0.05）。结论：不同调节状态下透镜形态的测量是成功的，治疗后晶状体形态有明显变化。所得数据将用作有限元模型的输入，以系统地评估FS晶状体切开术治疗的效果，在未来的工作。
Purpose: According to Helmholtz, accommodation is based on the flexibility of the crystalline lens, which decreases with age, causing presbyopia. With femtosecond (fs)-lentotomy treatment, it is possible to restore the flexibility of presbyopic lenses. The efficiency of the treatment can be systematically evaluated using the finite element method based on experimental data. The purpose of this study was to quantify the shape change of ex vivo lenses in different accommodation states according to the fs-lentotomy treatment.Methods: Five lenses with ciliary body excised from ex vivo porcine eyes (age: approximately 6 months, exact age unknown) were stretched in an accommodation device before and after laser treatment. Depending on the accommodation state, the lens shape, reconstructed from lens thickness, diameter, and anterior and posterior curvature, was measured using optical coherence tomography (OCT). The complete lens shape was parameterized and each measured parameter was compared to the results of a control group (n= 5, age: approximately 6 months, exact age unknown) without treatment.Results: The amplitudes of the parameters thickness (+ 140%), diameter (+ 54%), and anterior radius of curvature (+ 57%) significantly increased after treatment (P< 0.05), and showed no significant change for the control group. By contrast, the amplitude of the posterior radius of curvature showed no change after treatment (P> 0.05).Conclusions: Measurement of the lens shape in different accommodation states was successful and showed significant changes after the treatment. The resulting data will be utilized as input for a finite element model to systematically evaluate the effect of fs-lentotomy treatment in future work.