New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds
New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds
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DOI：
10.3390/ijms20082031
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发表时间：
2019-04
影响因子：
5.6
通讯作者：
W. Xi;Zong-xiang Tang;Shuyao Tang;Zujun Yang;Jie Luo;S. Fu
中科院分区：
文献类型：
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作者：
W. Xi;Zong-xiang Tang;Shuyao Tang;Zujun Yang;Jie Luo;S. Fu
堇草已广泛用于改良小麦 (Triticum aestivum L.) 品种。使用寡核苷酸（oligo）作为探针的非变性荧光原位杂交（ND-FISH）技术为识别小麦背景中的外来染色体提供了一种便捷有效的方法。然而，缺乏合适的 ND-FISH 阳性寡核苷酸探针来区分Thinopyrum 染色体和小麦。根据已发表的Thinopyrum ponticum (Th. ponticum) 特异性重复序列设计了两种寡核苷酸探针Oligo-B11 和Oligo-pThp3.93。 Oligo-B11和Oligo-pThp3.93均可用于ND-FISH分析，可以替代传统的GISH和FISH来区分Th的某些染色体。埃隆加图姆，Th。中间体和Th。 ponticum 在小麦背景中。这两种寡核苷酸探针为未来小麦育种计划中堇草属种质资源的利用提供了便捷的途径。
Thinopyrum has been widely used to improve wheat (Triticum aestivum L.) cultivars. Non-denaturing fluorescence in situ hybridization (ND-FISH) technology using oligonucleotides (oligo) as probes provides a convenient and efficient way to identify alien chromosomes in wheat backgrounds. However, suitable ND-FISH-positive oligo probes for distinguishing Thinopyrum chromosomes from wheat are lacking. Two oligo probes, Oligo-B11 and Oligo-pThp3.93, were designed according to the published Thinopyrum ponticum (Th. ponticum)-specific repetitive sequences. Both Oligo-B11 and Oligo-pThp3.93 can be used for ND-FISH analysis and can replace conventional GISH and FISH to discriminate some chromosomes of Th. elongatum, Th. intermedium, and Th. ponticum in wheat backgrounds. The two oligo probes provide a convenient way for the utilization of Thinopyrum germplasms in future wheat breeding programs.