Effects of 6-(Methylsulfinyl)hexyl Isothiocyanate Ingestion on Muscle Damage after Eccentric Exercise in Healthy Males: A Pilot Placebo-Controlled Double-Blind Crossover Study
Effects of 6-(Methylsulfinyl)hexyl Isothiocyanate Ingestion on Muscle Damage after Eccentric Exercise in Healthy Males: A Pilot Placebo-Controlled Double-Blind Crossover Study
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DOI：
10.1080/19390211.2021.1912244
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发表时间：
2021-04-04
影响因子：
2.5
通讯作者：
Takahashi, Hideyuki
中科院分区：
文献类型：
--
作者：
Tanabe, Yoko;Akazawa, Nobuhiko;Takahashi, Hideyuki
动物研究表明，6-（Methylsulfinyl）hexyl isothiocyanate（6-MSITC）是日本辛辣调味料芥末中的主要生物活性化合物，具有抑制钙蛋白酶-1（一种蛋白酶）活化的作用。钙蛋白酶活性的增加会导致离心运动后持续的力量损失。在人类中，6-MSITC摄入是否会调节离心运动后的钙蛋白酶和/或肌肉损伤反应仍有待确定。我们进行了一项随机、双盲、交叉设计的研究，其中8名健康的年轻男性被随机分配从运动前1天至运动后4天摄入9 mg/天的6-MSITC或安慰剂（使用等速测力计进行手肘屈肌的30次最大等速离心收缩）。评估钙蛋白酶-1浓度、炎症和肌肉损伤标志物（肌酸激酶活性、尿肌联蛋白浓度、肌肉力量、活动度、肌肉酸痛和横向松弛时间）。离心运动后的血浆钙蛋白酶-1浓度在安慰剂和6-MSITC治疗的条件下相似。相对于安慰剂治疗条件下的患者，所有肌肉损伤和炎症标志物均未受到6-MSITC的影响。我们的研究结果表明，6-MSITC对离心运动后测量的血浆钙蛋白酶-1浓度和肌肉损伤和炎症标志物没有影响。
An animal study demonstrated that 6-(Methylsulfinyl)hexyl isothiocyanate (6-MSITC), a major bioactive compound in Japanese pungent spice wasabi, has an action of inhibiting the activation of calpain-1 (a protease). Increases in calpain activity can cause continual strength loss after eccentric exercise. It remains to be determined in humans whether 6-MSITC intake would modulate calpain and/or muscle damage responses after eccentric exercise. We performed a randomized, double-blind, crossover design study wherein eight healthy young males were randomly assigned to ingest 9 mg/day of 6-MSITC or placebo from 1 day before exercise to 4 days after exercise (30 maximal isokinetic eccentric contractions of the elbow flexors using an isokinetic dynamometer). Calpain-1 concentration, inflammatory and muscle damage markers (creatine kinase activity, urinary titin concentration, muscle strength, range of motion, muscle soreness and transverse relaxation time) were assessed. Plasma calpain-1 concentration after eccentric exercise was similar between the placebo- and 6-MSITC-treated conditions. All muscle damage and inflammatory markers were not affected by 6-MSITC relative to those in the placebo-treated condition. Our results suggest that 6-MSITC has no effect on plasma calpain-1 concentration and muscle damage and inflammatory markers measured after eccentric exercise.