An evaluation of the RIDASCREEN and IDEIA enzyme immunoassays and the RIDAQUICK immunochromatographic test for the detection of norovirus in faecal specimens
An evaluation of the RIDASCREEN and IDEIA enzyme immunoassays and the RIDAQUICK immunochromatographic test for the detection of norovirus in faecal specimens
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DOI：
10.1016/j.jcv.2010.08.004
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发表时间：
2010-12-01
影响因子：
8.8
通讯作者：
Cuevas, Luis E.
中科院分区：
文献类型：
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作者：
Kirby, Andrew;Gurgel, Ricardo Q.;Cuevas, Luis E.
工作背景：通过ELISA和免疫层析方法检测诺如病毒可促进对与诺如病毒胃肠炎相关的全球疾病负担的流行病学研究，并提供检测诺如病毒感染的快速方法。评价新型RIDASCREEN诺如病毒ELISA（第三代）和RIDAQUICK诺如病毒免疫层析检测，对巴西急性胃肠炎儿童的样本进行检测，研究设计：使用逆转录酶PCR（研究参比标准品）检测726份标本中是否存在诺如病毒。结果：RIDASCREEN检测诺如病毒的敏感性为63%（95% CI：53-72%），RIDAQUICK为69%（95% CI：58-78%），特异性均>98%。IDEIA的敏感性为45%（95% CI：35-55%），显著低于RIDASCREEN和RIDAQUICK（p
Background: The detection of norovirus by ELISA and immunochromatographic methods may facilitate epidemiological studies into the global disease burden associated with norovirus gastroenteritis and provide a quick method of testing for norovirus infection.Objectives: To evaluate the new RIDASCREEN norovirus ELISA (3rd generation) and RIDAQUICK norovirus immunochromatographic test on a collection of samples from Brazilian children with acute gastroenteritis, and compare them against the established 2nd generation IDEIA norovirus assay.Study design: Reverse transcriptase PCR, the study reference standard, was used to test 726 specimens for the presence of norovirus. All 96 norovirus positive samples and a systematic selection of negative samples were tested by RIDASCREEN, RIDAQUICK and IDEIA norovirus tests.Results: The sensitivity of RIDASCREEN for the detection of norovirus was 63% (95% CI: 53-72%) and RIDAQUICK 69% (95% CI: 58-78%); both were >98% specific. The IDEIA had a sensitivity of 45% (95% CI: 35-55%), significantly lower than RIDASCREEN and RIDAQUICK (p