Low Counts of γδ T Cells in Peritumoral Liver Tissue are Related to More Frequent Recurrence in Patients with Hepatocellular Carcinoma after Curative Resection
Low Counts of γδ T Cells in Peritumoral Liver Tissue are Related to More Frequent Recurrence in Patients with Hepatocellular Carcinoma after Curative Resection
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DOI：
10.7314/apjcp.2014.15.2.775
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发表时间：
2014-01-01
影响因子：
--
通讯作者：
Qiu, Shuang-Jian
中科院分区：
文献类型：
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作者：
Cai, Xiao-Yan;Wang, Jia-Xing;Qiu, Shuang-Jian
目的：TCR-γ-δ + T细胞（γ δ T细胞）是一种能够识别和清除肿瘤细胞的非常规T淋巴细胞。我们以前的研究表明，γ δ T细胞的浸润和功能在肝细胞癌（HCC）中明显减弱。然而，其预后价值尚未明确。研究方法：在HCC患者队列（n = 342）中，通过免疫组织化学（IHC）确定γ δ T细胞与临床结局之间的关联。结果：免疫组化显示肿瘤组织中γ δ T细胞的浸润较成对的瘤周组织减少。瘤周γ δ T细胞计数与肿瘤大小呈负相关（P = 0.005）。生存分析显示，在单因素分析中，瘤周γ δ T细胞水平与复发时间（TTR）和总生存期（OS）相关（分别为P = 0.010和P = 0.036），在多因素分析中与TTR相关（P = 0.014，H.R. [95% CI] = 0.682 [0.502-0.927]）。此外，肿瘤周围γ δ T细胞的水平显示了巴塞罗那临床肝癌（BCLC）A期患者中TTR的独立预后价值（P = 0.038，H.R. [95% CI] = 0.727 [0.537-0.984]）。然而，肿瘤γ δ T细胞对HCC患者的TTR或OS没有显示独立的预后价值。结论：肿瘤周围肝组织中γ δ T细胞的低计数与HCC的较高复发率相关，并且可以预测术后复发，特别是在早期HCC患者中。
Objectives: TCR- gamma-delta + T cells (gamma delta T cells) are non-conventional T lymphocytes that can recognize and eradicate tumor cells. Our previous studies showed that infiltration and function of gamma delta T cells were substantially attenuated in hepatocellular carcinoma (HCC). However, their prognostic value was not clarified. Methods: The association between gamma delta T cells and the clinical outcomes was determined by immunohistochemistry (IHC) in a HCC patient cohort (n = 342). Results:Immunohistochemistry showed decreased infiltration of gamma delta T cells in tumoral tissues compared with paired peritumoral tissues. The counts of gamma delta T cells in peritumoral tissues were negatively correlated with tumor size (P = 0.005). Survival analysis showed that the levels of peritumoral gamma delta T cells were related to both time to recurrence (TTR) and overall survival (OS) (P = 0.010 and P = 0.036, respectively) in univariate analysis, and related to TTR in multivariate analysis (P = 0.014, H.R. [95% CI] = 0.682 [0.502-0.927]). Furthermore, the level of peritumoral gamma delta T cells showed independent prognostic value for TTR in Barcelona Clinic Liver Cancer (BCLC) stage A patients (P = 0.038, H.R. [95% CI] = 0.727 [0.537-0.984]). However, tumoral gamma delta T cells did not show independent prognostic value for either TTR or OS in HCC patients. Conclusions: Low counts of gamma delta T cells in peritumoral liver tissue are related to a higher incidence of recurrence in HCC and can predict postoperative recurrence, especially in those with early-stage HCC.