Global analysis of gene activity during Arabidopsis seed development and identification of seed-specific transcription factors
Global analysis of gene activity during Arabidopsis seed development and identification of seed-specific transcription factors
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DOI：
10.1073/pnas.1003530107
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发表时间：
2010-05-04
影响因子：
11.1
通讯作者：
Goldberg, Robert B.
中科院分区：
文献类型：
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作者：
Le, Brandon H.;Cheng, Chen;Goldberg, Robert B.
大多数负责控制种子发育的转录因子(TF)尚不清楚。为了确定在种子发育的特定阶段表达的转录因子基因，包括那些种子特有的基因，我们使用Affymetrix GeneChips来描述从受精到成熟以及在植物生命周期的其他时期种子中活跃的拟南芥基因。种子基因组与受精前胚珠、萌发幼苗以及成熟植株的叶、根、茎和花蕾中表达的基因进行了比较。种子中的大多数活性基因在种子发育的所有阶段都是共有的，尽管基因活性发生了显著的数量变化。种子发育的每个阶段都有一个小的基因组，它要么是基因芯片水平上特有的，要么是相对于其他阶段活跃的基因上调的，包括那些编码TF的基因。我们鉴定了289个种子特异基因，其中48个编码转录因子。其中7个种子特异的转录因子基因是已知的种子发育调节基因，包括叶状子叶基因LEC1、LEC1-like、LEC2和FUS3。其余的代表了不同类别的转基因作物，它们在种子发育中的作用未知。启动子-β-葡萄糖醛酸酶(GUS)融合实验和种子mRNA定位基因芯片数据集表明，种子特异的TF基因在种子不同发育时期的不同隔室和组织中都有活性。总的来说，这些种子特有的转铁蛋白基因应该有助于识别对种子发育规划至关重要的调控网络。
Most of the transcription factors (TFs) responsible for controlling seed development are not yet known. To identify TF genes expressed at specific stages of seed development, including those unique to seeds, we used Affymetrix GeneChips to profile Arabidopsis genes active in seeds from fertilization through maturation and at other times of the plant life cycle. Seed gene sets were compared with those expressed in prefertilization ovules, germinating seedlings, and leaves, roots, stems, and floral buds of the mature plant. Most genes active in seeds are shared by all stages of seed development, although significant quantitative changes in gene activity occur. Each stage of seed development has a small gene set that is either specific at the level of the GeneChip or up-regulated with respect to genes active at other stages, including those that encode TFs. We identified 289 seed-specific genes, including 48 that encode TFs. Seven of the seed-specific TF genes are known regulators of seed development and include the LEAFY COTYLEDON (LEC) genes LEC1, LEC1-LIKE, LEC2, and FUS3. The rest represent different classes of TFs with unknown roles in seed development. Promoter-beta-glucuronidase (GUS) fusion experiments and seed mRNA localization GeneChip datasets showed that the seed-specific TF genes are active in different compartments and tissues of the seed at unique times of development. Collectively, these seed-specific TF genes should facilitate the identification of regulatory networks that are important for programming seed development.