Effect of water-immersion restraint stress on tryptophan catabolism through the kynurenine pathway in rat tissues
Effect of water-immersion restraint stress on tryptophan catabolism through the kynurenine pathway in rat tissues
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DOI：
10.1007/s12576-016-0467-y
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发表时间：
2017-05-01
影响因子：
2.3
通讯作者：
Saito, Kuniaki
中科院分区：
文献类型：
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作者：
Ohta, Yoshiji;Kubo, Hisako;Saito, Kuniaki
本研究旨在阐明水浸束缚应激（WIRS）通过犬尿氨酸（Kyn）途径对大鼠组织中色氨酸（Trp）催化活性的影响。WIRS（+WIRS）处理6 h大鼠肝脏组织中色氨酸2，3-双加氧酶（TDO）和吲哚胺2，3-双加氧酶（IDO）活性增加，TDO和IDO 1（哺乳动物中两种IDO同工酶之一）mRNA表达水平增加，Trp含量降低，Kyn含量增加。+WIRS大鼠肾脏中的TDO和IDO活性不变，大脑中的TDO活性降低，IDO活性不变，肺和脾脏中的IDO活性不变，所有这些组织中的Kyn含量增加。用糖皮质激素拮抗剂RU 486预处理应激大鼠，减弱了增加的TOD活性，但不增加IDO活性，部分恢复了肝脏中减少的Trp和增加的Kyn含量。这些结果表明，WIRS通过诱导IDO 1和TDO来增强大鼠肝Trp催化剂。
The aim of this study was to clarify the effect of water-immersion restraint stress (WIRS) on tryptophan (Trp) catabolism through the kynurenine (Kyn) pathway in rat tissues. The tissues of rats subjected to 6 h of WIRS (+WIRS) had increased tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase (IDO) activities and increased TDO and IDO1 (one of two IDO isozymes in mammals) mRNA expression levels, with decreased Trp and increased Kyn contents in the liver. +WIRS rats had unchanged TDO and IDO activities in the kidney, decreased TDO activity and unchanged IDO activity in the brain, and unchanged IDO activity in the lung and spleen, with increased Kyn content in all of these tissues. Pretreatment of stressed rats with RU486, a glucocorticoid antagonist, attenuated the increased TOD activity, but not the increased IDO activity, with partial recoveries of the decreased Trp and increased Kyn contents in the liver. These results indicate that WIRS enhances hepatic Trp catabolism by inducing both IDO1 and TDO in rats.