Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery.
Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery.
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DOI：
10.1038/srep46183
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发表时间：
2017-04-07
影响因子：
4.6
通讯作者：
Yan Y
中科院分区：
文献类型：
--
作者：
Bian Y;Deng X;Yan X;Zhou J;Yuan L;Yan Y
在这项研究中，我们首次对短柄草（Brachypodium distachyon L.）的根和叶进行了干旱响应和干旱恢复的生理和蛋白质组学综合分析。干旱胁迫导致叶片卷曲，根尖颜色变深，部分生理参数发生显著变化。二维差异凝胶电泳（2D-DIGE）鉴定出78个和98个差异积累蛋白（DAP）点，分别代表68个和73个响应干旱胁迫和/或恢复的独特蛋白质。根与叶蛋白质组在光合作用、能量代谢和蛋白质代谢方面的差异最为显著。特别是，一些参与能量和蛋白质代谢的DAPs在根和叶中的积累模式截然不同。蛋白质-蛋白质相互作用（PPI）分析揭示了根系和叶片在干旱胁迫和干旱恢复过程中产生协同响应的复杂蛋白质相互作用网络。利用实时定量聚合酶链反应（qRT-PCR）进行转录分析，证实了PPI网络中涉及的关键蛋白的差异表达。我们的综合生理和蛋白质组学分析为短柄茅根和叶片中参与干旱响应和干旱恢复的协同网络提供了证据。
In this study, we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought, using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling, root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves, respectively. Differences between the root and leaf proteome were most marked for photosynthesis, energy metabolism, and protein metabolism. In particular, some DAPs involved in energy and protein metabolism had contrasting accumulation patterns in roots and leaves. Protein-protein interaction (PPI) analysis of roots and leaves revealed complex protein interaction networks that can generate synergistic responses to drought stress and during recovery from drought. Transcript analysis using quantitative real-time polymerase chain reaction (qRT-PCR) validated the differential expression of key proteins involved in the PPI network. Our integrated physiological and proteomic analysis provides evidence for a synergistic network involved in responses to drought and active during recovery from drought, in Brachypodium roots and leaves.