Self-assembled pH-responsive hydrogels composed of the RATEA16 peptide.
Self-assembled pH-responsive hydrogels composed of the RATEA16 peptide.
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DOI：
10.1021/bm701143g
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发表时间：
2008-05
影响因子：
6.2
通讯作者：
Ying Zhao;H. Yokoi;Masayoshi Tanaka;T. Kinoshita;T. Tan
中科院分区：
文献类型：
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作者：
Ying Zhao;H. Yokoi;Masayoshi Tanaka;T. Kinoshita;T. Tan
肽RATEA16在生理条件下自发自组装成含水量极高（>99.5%（wt/vol））的高阶纳米纤维水凝胶。水凝胶可以经历从粘性溶液到弹性水凝胶并沉淀的pH可逆转变。超分子自组装和三相变是由疏水相互作用、分子间氢键以及吸引或排斥静电相互作用的组合驱动的。 CD 和 FTIR 数据证明，这些水凝胶富含 β-折叠纳米纤维。流变学测量表明，材料的粘弹性可以通过环境 pH 值和肽浓度进行调节。水凝胶的储能模量随着肽浓度的增加而增加，并且自组装水凝胶能够从机械故障中恢复。 AFM 图像显示，弹性归因于由纤维自组装体组成的网络纳米结构。水凝胶有望用于各种可能的生物医学应用，包括药物输送。
Peptide RATEA16 spontaneously self-assembled into higher-order nanofiber hydrogels with extremely high water content (>99.5% (wt/vol)) under physiological condition. The hydrogels could undergo pH-reversible transitions from viscous solution to elastic hydrogel and to precipitate. The supramolecular self-assembly and the three phase transitions are driven by hydrophobic interactions, intermolecular hydrogen bonds, and a combination of attractive or repulsive electrostatic interactions. These hydrogels are rich in beta-sheet nanofibers, as demonstrated by CD and FTIR data. Rheological measurements reveal that the viscoelasticity of the material can be tuned by environmental pH and peptide concentration. The storage modulus of the hydrogels increases with increasing peptide concentration, and the self-assembled hydrogels are able to recover from mechanical breakdowns. AFM images show that the elasticity is attributed to a network nanostructure consisting of fibrous self-assemblies. The hydrogels are promising for a variety of possible biomedical applications, including drug delivery.