EFFECT OF PROTEIN ADSORPTION ON THE SURFACE-CHARGE CHARACTERISTICS OF ALUMINUM-CONTAINING ADJUVANTS
EFFECT OF PROTEIN ADSORPTION ON THE SURFACE-CHARGE CHARACTERISTICS OF ALUMINUM-CONTAINING ADJUVANTS
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DOI：
10.1016/0264-410x(94)90127-9
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发表时间：
1994-04-01
期刊：
影响因子：
5.5
通讯作者：
HEM, SL
中科院分区：
文献类型：
--
作者：
ALSHAKHSHIR, R;REGNIER, F;HEM, SL
研究了吸附牛血清白蛋白和溶菌酶两种模型蛋白对含铝疫苗佐剂零电点的影响。在生理pH条件下，氢氧化铝佐剂(零电点为11.1)对带负电的白蛋白(等电点为5.0)的吸附使其零电荷点降低。相反，带负电的磷酸铝佐剂(零电荷点=5.0)对带正电的溶菌酶(等电点为9.6)的吸附导致零电荷点的增加。在蛋白质覆盖率较低时，含铝佐剂的表面电荷特性占主导地位。相反，在蛋白质覆盖率较高时，吸附蛋白质的表面电荷特征占主导地位。因此，在制备疫苗时，应考虑抗原-佐剂复合体的物理化学性质，而不是仅考虑佐剂的物理化学性质。
The effect of adsorbing two model proteins, bovine serum albumin and lysozyme, on the point of zero charge of aluminium-containing vaccine adjuvants was studied. At physiological pH, the adsorption of the negatively charged albumin (isoelectric point = 5.0) by aluminium hydroxide adjuvant (point of zero charge=11.1) resulted in a decrease in the point of zero charge. In contrast, the adsorption of positively charged lysozyme (isoelectric point=9.6) by the negatively charged aluminium phosphate adjuvant (point of zero charge=5.0) resulted in an increase in the point of zero charge. The surface charge characteristics of the aluminium-containing adjuvant dominated at low protein coverage. In contrast, the surface charge characteristics of the adsorbed protein dominated at high protein coverage. Therefore, the physicochemical properties of the antigen-adjuvant complex and not the adjuvant alone should be considered during vaccine preparation.