Severe H7N9 infection is associated with decreased antigen-presenting capacity of CD14+ cells.
Severe H7N9 infection is associated with decreased antigen-presenting capacity of CD14+ cells.
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DOI：
10.1371/journal.pone.0092823
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Diao H;Cui G;Wei Y;Chen J;Zuo J;Cao H;Chen Y;Yao H;Tian Z;Li L
H7N9人感染的暴发引起了全世界的关注，但感染患者的免疫学特征和不同结果的决定因素仍有待彻底了解。在这项研究中，23名H7N9感染患者被分为重度和轻度病例。我们发现重症患者通常是淋巴细胞减少，与轻型患者相比，T细胞、单核细胞和相关细胞因子水平显著降低。重症感染组CD14+细胞表面HLADR的表达明显低于轻度感染组(急性期分别为34.65±4.88vs.10.37±1.69，p<；0.001)。CD14+细胞表面HLA-DR的表达与H7N9感染严重程度呈负相关。此外，虽然两组单核细胞的吞噬能力相似，但严重感染患者的单核细胞抗原提呈能力较低。一些体外实验表明，抗原提呈功能受损与免疫刺激反应中T细胞的激活程度降低有关。我们目前的研究提示H7N9重症患者处于免疫低下状态，表现为普遍的淋巴细胞减少和抗原提呈能力低下，导致T细胞应答受损。此外，CD14+细胞的HLA-DR水平可能是预测H7N9疾病进展的潜在生物标志物。
The outbreak of H7N9 human infection has caused concern worldwide, but the immunological characteristics of infected patients and the determinants of diverse outcomes remain to be thoroughly understood. In this study, twenty-three patients with H7N9 infections were classified into severe and mild cases. We found that severe patients were commonly lymphopenic with significantly lower levels of T cells, monocytes and related cytokine levels compared to the mild cases. The expression of HLA-DR on CD14+ cells were significantly lower in the severe infection group compared to the mild group (in acute phase: 34.65±4.88 vs. 10.37±1.69, p<0.001). Importantly, the expression of HLA-DR on CD14+ cells was negatively correlated with H7N9 infection severity. Furthermore, although the phagocytosis capabilities of monocyte were similar between two groups, the monocytes of severe infection patients had a lower antigen-presenting capacity. And some in vitro experiments suggested that the impaired antigen-presenting function is associated with lower activation of T cells in responses to immune stimulation. Our present study suggested that the severe H7N9 patients were in a state of immune decrease which presented with general lymphopenia and low antigen-presenting capacity resulting in impaired T cell response. Additionally, HLA-DR levels of CD14+ cells may be a potential biomarker for predicting H7N9 disease progression.
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影响因子：
158.5
作者：
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DOI：
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DOI：
10.1152/ajpgi.00105.2005
发表时间：
2005-11-01
影响因子：
4.5
作者：
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通讯作者：
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