The effect of tumor necrosis factor/cachectin on follicle-stimulating hormone-induced aromatase activity in cultured rat granulosa cells.
The effect of tumor necrosis factor/cachectin on follicle-stimulating hormone-induced aromatase activity in cultured rat granulosa cells.
复制标题
肿瘤坏死因子/恶病素对培养的大鼠颗粒细胞中卵泡刺激素诱导的芳香酶活性的影响。
DOI：
10.1016/s0006-291x(88)81165-3
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发表时间：
1988
影响因子：
3.1
通讯作者：
Baird,A
中科院分区：
文献类型：
--
作者：
Emoto,N;Baird,A
利用[1β-3H]雄烯二酮中的3 H立体专一性转移到3 H2O中的方法，研究了肿瘤坏死因子（TNF）/恶病质素（Cachectin）对卵泡刺激素（FSH）诱导的大鼠颗粒细胞芳香化酶活性的影响。TNF（10 pg/ml ~ 10 ng/ml）呈浓度依赖性地抑制FSH（250 ng/ml）诱导的芳香化酶活性，10 ng/ml的TNF完全消除FSH诱导的芳香化酶活性。对TNF影响的时程分析表明，TNF对诱导的芳香化酶活性没有影响，但抑制FSH对该酶的进一步诱导。TNF（10 ng/ml）也抑制TGFβ（1 ng/ml）增强芳香化酶活性和增加孕酮合成的能力。因此，TNF是调节卵巢功能的一系列复杂蛋白质的组成部分，因此，可能在调节颗粒细胞中发挥生理作用。鉴于其与恶病质的相关性，它也可能在慢性疾病期间抑制生殖功能方面发挥病理生理作用。
We investigated the effects of tumor necrosis factor (TNF)/cachectin on follicle-stimulating hormone (FSH)-induced aromatase activity in cultured rat granulosa cells using the stereospecific transfer of3H from [1β-3H] androstenedione into3H2O. TNF (10 pg/ml - 10 ng/ml) inhibited FSH (250 ng/ml)-induced aromatase activity in a concentration-dependent manner, and 10 ng/ml of TNF completely abolished the FSH-induced aromatase activity. A time course analysis of the effects of TNF showed that TNF had no effect on induced aromatase activity, but inhibited the further induction of the enzyme by FSH. TNF (10 ng/ml) also inhibited the ability of TGFβ(1 ng/ml) to enhance aromatase activity and increase progesterone synthesis. Thus, TNF is a component of the complex array of proteins that modulate ovarian function and, as such, may play a physiological role in the regulation of the granulosa cell. In view of its association with cachexia, it may also play a pathophysiological role in the suppression of reproductive function during chronic illness.