Novel sesquiterpenoids isolated from Chimonanthus praecox and their antibacterial activities
Novel sesquiterpenoids isolated from Chimonanthus praecox and their antibacterial activities
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从腊梅中分离的新型倍半萜类化合物及其抗菌活性
DOI：
10.1016/s1875-5364(18)30100-6
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发表时间：
2018-08-01
影响因子：
4.6
通讯作者：
Pan Wei-Dong
中科院分区：
文献类型：
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作者：
Lou Hua-Yong;Zhang Yu;Pan Wei-Dong
从蜡梅（Chimonanthuspraecox Link）的乙酸乙酯提取物中分离得到4个新的倍半萜类化合物，即蜡梅醇A-D（化合物1-4）和4个已知化合物（5-8）。这些新化合物的结构进行了阐明的基础上，光谱技术（UV，IR，MS，和1D和2D NMR），并通过比较实验和计算的电子圆二色性（ECD）光谱，建立了它们的绝对构型。评价化合物1-8的抗微生物活性，并通过肉汤微量稀释法在96孔培养板中测定最小抑菌浓度（MIC）。化合物1、2和7对S. aureus（ATCC 6538）、E.大肠杆菌（ATCC 11775）和铜绿假单胞菌（ATCC 10145），MIC值为158-249 μ g.mL（-1）。化合物3-7对黄瓜枯萎病菌有一定的抑制活性。glabrata（ATCC 2001）和S.金黄色葡萄球菌（ATCC 43300），MIC值为128-197 μ g.mL（-1）。化合物1-4对沙门氏菌有一定的抑菌活性。金黄色葡萄球菌（ATCC 25923），MIC值为162-254 μ g.mL（-1）。本研究为今后评价这些化合物作为抗菌剂提供了基础。
In the present study, four new sesquiterpenoids, chimonols A-D (compounds 1-4), together with four known compounds (5-8) were isolated from the EtOAc extract of Chimonanthus praecox Link. The structures of these new compounds were elucidated on the basis of spectroscopic techniques (UV, IR, MS, and 1D and 2D NMR), and their absolute configurations were established by comparing experimental and calculated electronic circular dichroism (ECD) spectra. Compounds 1-8 were evaluated for antimicrobial activities and the minimum inhibitory concentrations (MICs) were determined by the broth microdilution method in 96-well culture plates. Compounds 1, 2, and 7 exhibited weak antibacterial effects for S. aureus (ATCC 6538), E. coli (ATCC 11775), and P. aeruginosa (ATCC 10145) with MIC values being 158-249 mu g.mL(-1). Compounds 3-7 showed activities against C. glabrata (ATCC 2001) and S. aureus (ATCC 43300) with MIC values being 128-197 mu g.mL(-1). Compounds 1-4 showed activity against S. aureus (ATCC 25923) with MIC values being 162-254 mu g.mL(-1). The present study provided a basis for future evaluation of these compounds as antibacterial agents.