Synaptic and morphologic properties in vitro of premotor rat nucleus tractus solitarius neurons labeled transneuronally from the stomach
Synaptic and morphologic properties in vitro of premotor rat nucleus tractus solitarius neurons labeled transneuronally from the stomach
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DOI：
10.1002/cne.10831
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发表时间：
2003-09-29
影响因子：
2.5
通讯作者：
Smith, BN
中科院分区：
文献类型：
--
作者：
Glatzer, NR;Hasney, CP;Smith, BN
大鼠孤束核（NTS）的神经元具有与其接收突触信息类型相关的形态学特征。这些特征已被描述为内脏感觉神经元，但未描述为运动前NTS神经元。采用全细胞膜片钳记录和脑干切片生物细胞素标记，观察大鼠尾侧NTS神经元的形态和突触特征。胃相关的运动前NTS神经元在接种胃壁后被鉴定为记录用跨神经元逆行病毒标记，报告增强的绿色荧光蛋白。基于体细胞面积和近端树突树突化的定量方面，确定了NTS神经元的三个形态组，这些测量在切片记录中是一致的。最常见的细胞类型（第一组）具有相对较小的体细胞和一到三个稀疏分支的树突，而其他组具有较大的体细胞和三个以上的树突，这些树突主要靠近（第二组）或远离（第三组）体细胞。电压钳记录显示，无论形态如何，所有神经元均存在自发兴奋性和抑制性突触后电流。胃相关的运动前NTS神经元构成三种形态类型中的两种（即I组和II组）。与未标记的神经元相比，这些细胞不太可能接受来自孤束的恒定潜伏期突触输入。这些结果驳斥了NTS神经元突触输入的一般模式取决于形态的假设。胃前运动神经元包括NTS形态类型的一个子集，其脏器感觉输入的组织尚未确定。(C) 2003 Wiley-Liss, Inc。
Neurons in the rat nucleus tractus solitarius (NTS) possess morphologic characteristics that have been correlated with the type of synaptic information they receive. These features have been described for viscerosensory neurons but not for premotor NTS neurons. The morphologic and synaptic features of neurons in the rat caudal NTS were assessed using whole-cell patch-clamp recordings and biocytin labeling in brainstem slices. Gastric-related premotor NTS neurons were identified for recording after inoculation of the stomach wall with a transneuronal retrograde viral label that reports enhanced green fluorescent protein. Three morphologic groups of NTS neurons were identified based on quantitative aspects of soma area and proximal dendritic arborization, measures that were consistent across slice recordings. The most common type of cell (group I) had relatively small somata and one to three sparsely branching dendrites, whereas the other groups had larger somata and more than three dendrites, which branched predominantly close to (group II) or distant from (group III) the soma. Voltage-clamp recordings revealed spontaneous excitatory and inhibitory postsynaptic currents in all neurons, regardless of morphology. Gastric-related premotor NTS neurons composed two of the three morphologic types (i.e., groups I and II). Compared with unlabeled neurons, these cells were less likely to receive constant-latency synaptic input from the tractus solitarius. These results refute the hypothesis that general patterns of synaptic input to NTS neurons depend on morphology. Gastric premotor neurons comprise a subset of NTS morphologic types, the organization of the viscerosensory input to which has yet to be defined. (C) 2003 Wiley-Liss, Inc.