Chronic Constriction Injury of the Rat's Infraorbital Nerve (IoN-CCI) to Study Trigeminal Neuropathic Pain
Chronic Constriction Injury of the Rat's Infraorbital Nerve (IoN-CCI) to Study Trigeminal Neuropathic Pain
复制标题
DOI：
10.3791/53167
复制
发表时间：
2015-09-01
影响因子：
1.2
通讯作者：
Hans, Guy H.
中科院分区：
文献类型：
--
作者：
Deseure, Kristof;Hans, Guy H.
动物模型是研究神经病理性疼痛的病理生理学和药理学的重要工具。本文描述了研究大鼠三叉神经病理性疼痛的手术和行为过程。为了满足三叉神经病理性疼痛综合征的特殊性，通过松散结扎神经对眶下神经（IoN）进行慢性压迫性损伤（CCI）。这里提出了一种眶内方法来暴露和结扎眶腔内的IoN。在IoN结扎后，大鼠表现出自发行为的变化和对von Frey毛发刺激的响应，这表明持续性疼痛和机械性异常性疼痛。在行为变化的发展过程中可以定义两个阶段。在IoN-CCI（阶段1）后的第一周期间，大鼠显示出增加的和不对称的面部梳理活动，即，主要针对神经损伤的IoN区域进行洗脸。在作为更一般的身体修饰行为的一部分的面部修饰行为和既不先于也不跟随身体修饰的面部修饰行为之间进行区分，所述面部修饰行为在很大程度上不受IoN-CCI影响，所述身体修饰在IoN-CCI之后显著增加。在此期间，对IoN区域的机械刺激的反应性降低。这种低反应性突然被极端的高反应性所取代，即使是非常弱的刺激强度也会引起伤害反应行为（第2阶段）。这些行为改变和报告的面部疼痛迹象之间的现象学相似性（即，对面部伤害性刺激的反应）表明在结扎的IoN区域中存在感觉迟钝/感觉异常和机械性异常性疼痛。
Animal models are important tools to study the pathophysiology and pharmacology of neuropathic pain. This manuscript describes the surgical and behavioral procedures to study trigeminal neuropathic pain in rats. To meet the specificity of trigeminal neuropathic pain syndromes, the infraorbital nerve (IoN) is subjected to a chronic constriction injury (CCI) by loosely ligating the nerve. An intra-orbital approach is presented here to expose and ligate the IoN in the orbital cavity. After IoN ligation, rats exhibit changes in spontaneous behavior and in response to von Frey hair stimulation that are indicative of persistent pain and mechanical allodynia. Two phases can be defined in the development of the behavioral changes. During the first week following IoN-CCI (phase 1), rats show an increased and asymmetric face grooming activity, i.e., with face wash strokes primarily directed to the nerve-injured IoN territory. A distinction is made between face grooming behavior that is part of a more general body grooming behavior, which remains largely unaffected by IoN-CCI, and face grooming that is neither preceded nor followed by body grooming, which is significantly increased after IoN-CCI. During this period, responsiveness to mechanical stimulation of the IoN territory is reduced. This hyporesponsiveness is abruptly replaced by an extreme hyperresponsiveness whereby even very weak stimulus intensities provoke nocifensive behavior (phase 2). The phenomenological similarities between these behavioral alterations and reported signs of facial pain (i.e., responses to noxious stimulation of the face) suggest the presence of dysesthesia/paresthesia and mechanical allodynia in the ligated IoN territory.