Differential induction of protein kinase C isoforms at the cardiac hypertrophy stage and congestive heart failure stage in Dahl salt-sensitive rats
Differential induction of protein kinase C isoforms at the cardiac hypertrophy stage and congestive heart failure stage in Dahl salt-sensitive rats
复制标题
DOI：
10.1291/hypres.26.421
复制
发表时间：
2003-05-01
影响因子：
5.4
通讯作者：
Umemura, S
中科院分区：
文献类型：
--
作者：
Koide, Y;Tamura, K;Umemura, S
蛋白激酶C（PKC）的几种异构体可能在细胞信号通路中发挥重要作用。最近的研究表明，PKC在心肌肥厚和心力衰竭的发展中起着关键的亚型特异性作用。本研究的目的是研究PKC异构体在心肌肥厚和心力衰竭模型中的表达谱。我们通过Western印迹分析检测了Dahl盐敏感大鼠心肌肥厚阶段和心力衰竭阶段单个PKC亚型的心脏表达。在心肌肥厚期和心力衰竭期，所有PKC亚型的水平均升高，但在心力衰竭期不同PKC亚型的升高模式不同。PKCalpha、β和δ的表达在心肌肥厚阶段增加，并在心力衰竭阶段保持升高。另一方面，PKC β和非典型PKC（aPKC）的表达在心肌肥厚阶段增加，但这种增加在充血性心力衰竭阶段趋于下降。这些结果表明，有两组PKC亚型。一些报道表明，PKCalpha、β和δ参与心脏肥大和心力衰竭的发展，并且PKCa在生理性肥大反应和心脏保护作用中起作用。这些事实表明，在心脏中表达的所有PKC同工型（PKCalpha、β、δ、α和aPKC）在心脏肥大阶段可能具有相似的功能，但两组PKC同工型（PKCalpha、β、δ和PKC β、aPKC）在充血性心力衰竭阶段具有不同的功能。
Several protein kinase C (PKC) isoforms may play important roles in cellular signaling pathways. Recent reports have suggested that PKC plays critical isoform-specific roles in the development of cardiac hypertrophy and heart failure. The purpose of the present study was to examine the expression profiles of PKC isoforms in models of cardiac hypertrophy and heart failure. We examined the cardiac expression of individual PKC isoforms at the cardiac hypertrophy stage and the heart failure stage in Dahl salt-sensitive rats by Western blot analysis. The levels of all PKC isoforms increased at the cardiac hypertrophy stage and the heart failure stage, but the pattern of increase differed among PKC isoforms at the heart failure stage. The expressions of PKCalpha, beta, and delta increased at the cardiac hypertrophy stage and remained elevated at the heart failure stage. On the other hand, the expression of PKCepsilon and atypical PKCs (aPKCs) increased at the cardiac hypertrophy stage, but this increase tended to decline at the congestive heart failure stage. These results suggest that there are two groups of PKC isoforms. Several reports have shown that PKCalpha, beta, and delta are involved in the development of cardiac hypertrophy and heart failure, and that PKCepsilon plays a role in the physiological hypertrophic responses and cardioprotective actions. These facts suggest that all PKC isoforms (PKCalpha, beta, delta, epsilon, and aPKCs) expressed in the heart may have similar functions at the cardiac hypertrophy stage, but that two groups of PKC isoforms (PKCalpha, beta, delta, and PKCepsilon, aPKCs) have different functions at the congestive heart failure stage.