Identification of a novel polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) gene required for the biosynthesis of cyclopiazonic acid in Aspergillus oryzae
Identification of a novel polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) gene required for the biosynthesis of cyclopiazonic acid in Aspergillus oryzae
复制标题
DOI：
10.1016/j.fgb.2008.09.006
复制
发表时间：
2008-12-01
影响因子：
3
通讯作者：
Koyama, Yasuji
中科院分区：
文献类型：
--
作者：
Tokuoka, Masafumi;Seshime, Yasuyo;Koyama, Yasuji
环匹阿尼酸（CPA）是由青霉属和曲霉属的几种菌株产生的真菌毒素。用于发酵食品的曲霉菌株不产生CPA;然而，几种野生型A。菌株产生CPA。在这里，我们通过比较CPA生产菌株（A. AMBNRC 4177）与非产酶菌株（A. R1B40）。NBRC 4177在RIB 40中端粒重复序列的区域之外有一个额外的17-18-kb的序列，这个额外的区域包含PKS-NRPS基因的31区，而RIB 40只有PKS-NRPS基因的5'区。NBRC 4177中PKS-NRPS基因的基因破坏导致CPA产生的消除。因此，PKS-NRPS基因是CPA生物合成所必需的，该基因的截短被认为是A. 40. baby (C)2008年爱思唯尔公司All rights reserved.
Cyclopiazonic acid (CPA) is a mycotoxin produced by several strains of Penicillium and Aspergillus species. Aspergillus oryzae strains used in fermented foods do not produce CPA; however, several wild-type A. oryzae strains produce CPA. Here, we identified a novel polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) gene involved in CPA production by comparing the telomere-adjacent region of a CPA-producing strain (A. oryzae NBRC 4177) with that of a nonproducing strain (A. oryzae R1B40). NBRC 4177 has an additional 17-18-kb sequence beyond the region corresponding to the telomere repeat in RIB40 and this additional regions contains 31 region of the PKS-NRPS gene, while RIB40 has only the 5' region of the PKS-NRPS gene. Gene disruption of the PKS-NRPS gene in NBRC 4177 resulted in elimination of CPA production. Thus, the PKS-NRPS gene is required for CPA biosynthesis, and the truncation of this gene is presumed as one of the determinants of CPA nonproductivity in A. oryzae RIB40. (C) 2008 Elsevier Inc. All rights reserved.