Blood serum branched chain amino acids and tryptophan modifications in horses competing in long-distance rides of different length
Blood serum branched chain amino acids and tryptophan modifications in horses competing in long-distance rides of different length
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DOI：
10.1111/j.1439-0396.2004.00493.x
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发表时间：
2004-04-01
影响因子：
2.7
通讯作者：
Caola, G
中科院分区：
文献类型：
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作者：
Assenza, A;Bergero, D;Caola, G
在长距离运动中，支链氨基酸(BCAA)的分解代谢会导致血液中色氨酸/BCAA比值的增加，并导致所有人过早地出现“中枢性疲劳”。基于这些考虑，我们研究了30匹参加距离从20到72公里的耐力赛马的血清支链氨基酸和色氨酸(Try)水平的变化。所有马匹的血液样本都是在骑行结束前和结束后采集的。对样本进行了亮氨酸(Leu)、缬氨酸(Val)、异亮氨酸(Iso)和Try水平的分析。数据处理采用单因素方差分析，以抽样时刻和游乐设施为因素，采用LSD后置检验。在不同的骑行距离中，亮氨酸、Val、Iso、Try、Try/BCAA比率有显著差异；在比赛开始和结束时采集的样本中，Val和Leu也有相同的趋势。观察到的主要影响是除了72公里的骑行外，所有比赛的支链氨基酸水平都增加了；对于Try，除了50公里的骑行外，所有比赛的支链氨基酸水平都有显著的增加。Try/BCAA比值在20公里和50公里比赛中下降，在其他比赛中上升。这些数据证实，长距离运动涉及支链氨基酸的动员。支链氨基酸的利用在长时间运动中似乎很重要：在72公里的骑行中，我们观察到支链氨基酸血清水平下降，而Try的主要作用是它的增加，导致Try/BCAA比率上升。
During long-distance exercise, branched-chain amino acid (BCAA) catabolism could lead to an increase in the blood tryptophan/BCAA ratio and all early onset of 'central fatigue'. Based on these considerations, we studied the modifications of blood serum BCAA and tryptophan (Try) levels in 30 endurance horses competing in rides varying in distance from 20 to 72 km. From all horses, blood samples were drawn Just before and just after the end of the ride. Samples were analysed for their leucine (Leu), valine (Val), isolcucine (Iso) and Try levels. Data were processed by ANOVA, using sampling moment and ride as factors, and by LSD post hoc test. Significant differences were recorded among the different distance rides for Leu, Val, Iso, Try, Try/BCAA ratio; the same trend was recorded between samples taken at the start and the end of the race for Val and Leu. The main effect observed was an increase of BCAA levels for all rides, except the 72-km ride; for Try, a significant increase was present in all races, except the 50-km ride. The Try/BCAA ratio decreased in 20- and 50-km races and increased in the others. These data confirm that long-distance exercise involves a mobilization of BCAA. The utilization of BCAA seems to be important in prolonged exercise: in the 72-km ride, we observed a decrease in BCAA blood serum levels, while a major role of Try was indicated by its increase, resulting in a rise of the Try/ BCAA ratio.