Morphological Differentiation of Neurons on Microtopographic Substrates Fabricated by Rolled-Up Nanotechnology
Morphological Differentiation of Neurons on Microtopographic Substrates Fabricated by Rolled-Up Nanotechnology
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DOI：
10.1002/adem.201080023
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发表时间：
2010-09-01
影响因子：
3.6
通讯作者：
Schmidt, Oliver G.
中科院分区：
文献类型：
--
作者：
Schulze, Sabine;Huang, Gaoshan;Schmidt, Oliver G.
利用传统光刻技术、电子束沉积技术和化学蚀刻技术，可以很容易地批量生产透明的卷曲微管阵列。在这里，我们在各种微管基质上培养原代小鼠运动神经元和永生化CAD细胞（一种源自中枢神经系统的细胞系），以研究地形表面特征对这些细胞生长和分化行为的影响。我们的研究结果表明，微管芯片不仅支持两种细胞类型的生长，而且还提供了一个定义良好的、几何限制的3D细胞培养支架。引人注目的是，我们的微模式充当了轴突引导的平台，突出的细胞延伸与微管的方向对齐，形成复杂的方形网格状神经突网络。我们的实验开辟了一种具有成本效益和生物相容性的方法，可以在先进的微/纳米结构的背景下分析单细胞行为，并具有从神经突保护研究到细胞传感器开发的各种生物学应用。
Arrays of transparent rolled-up microtubes can easily be mass-produced using a combination of conventional photolithography, electron beam depositioning, and chemical etching techniques. Here, we culture primary mouse motor neurons and immortalised CAD cells, a cell line derived from the central nervous system, on various microtube substrates to investigate the influence of topographical surface features on the growth and differentiation behaviour of these cells. Our results indicate that the microtube chips not only support growth of both cell types but also provide a well-defined, geometrically confined 3D cell culture scaffold. Strikingly, our micropatterns act as a platform for axon guidance with protruding cell extensions aligning in the direction of the microtubes and forming complex square-shaped grid-like neurite networks. Our experiments open up a cost-efficient and bio-compatible way of analysing single cell behaviour in the context of advanced micro-/nanostructures with various biological applications ranging from neurite protection studies to cell sensor development.