Anti-hepatitis B virus activity of swertisin isolated from Iris tectorum Maxim
Anti-hepatitis B virus activity of swertisin isolated from Iris tectorum Maxim
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从鸢尾中分离出的獐牙菜素的抗乙型肝炎病毒活性
DOI：
10.1016/j.jep.2020.112787
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发表时间：
2020
影响因子：
5.4
通讯作者：
Chen Juan
中科院分区：
文献类型：
--
作者：
Xu Hong-Yan;Ren Ji-Hua;Su Yu;Ren Fang;Zhou Yu-Jiao;Jiang Hui;Cheng Sheng-Tao;Zhang Chuan-Rui;Chen Juan
鸢尾花（鸢尾花）是一种常见的中药，用于治疗肝脏相关疾病。然而，抗hbv活性ofI。菌株及其分离株尚未得到系统的研究。研究目的：筛选活性成分。系统评价其抗hbv活性。材料与方法在本研究中，从植物中提取了一系列化合物。对其抑制HBV复制的能力进行了评估。獐牙菜素对HBV复制有明显的抑制作用。然后，综合评价不同浓度獐牙菜素对HepG2.2.15 细胞和HBV感染的HepG2-NTCP细胞HBsAg、HBeAg和HBV DNA水平的抑制作用。此外，在HBV转基因小鼠模型中证实了獐牙菜素的抗HBV作用。结果獐牙菜素对HepG2.2.15 细胞和HBV感染的HepG2-NTCP细胞的HBsAg、HBeAg和HBV DNA水平有较强的抑制作用，且呈剂量依赖性。此外，在HBV转基因小鼠模型中，獐牙菜素对HBV复制有明显的抑制作用，与ETV联用后抑制作用增强。结论在体外和体内实验中，我们发现獐牙菜素可以抑制HBeAg和HBsAg的产生，以及抑制HBV DNA的产生。本研究表明，我们可能从中药中分离出一种具有有效抗hbv功能的新化合物。
Ethnopharmacological relevanceIris tectorumMaxim (I. tectorum, Yuan Wei in Chinese) is a common and traditional Chinese medicinal herb that be used to treat liver-related diseases. However, the anti-HBV activity ofI. tectorumand its isolates has not been systemically studied.Aim of the studyTo screen the active part ofI. tectorumand systemically evaluate their anti-HBV activity.Materials and methodsIn this study, a series of compounds fromI. tectorumwere evaluated for their ability to inhibit HBV replication. Swertisin showed a significant inhibitory function on HBV replication. Then, the suppression effect of different concentrations of swertisin in HBsAg, HBeAg and HBV DNA level in HepG2.2.15 cells and HBV-infected HepG2-NTCP cells were comprehensive evaluated, respectively. Moreover, the anti-HBV effects of swertisin were confirmed in HBV transgenic mice model.ResultsAmong these compounds, swertisin strongly inhibited the HBsAg, HBeAg and HBV DNA level in a dose-dependent manner in HepG2.2.15 cells and HBV-infected HepG2-NTCP cells. Furthermore, swertisin showed a significant inhibition role on HBV replication in HBV transgenic mice model, the inhibition effect of which was enhanced when combined with ETV.ConclusionsWe have identified that swertisin can inhibit HBeAg and HBsAg production, as well as HBV DNA in vitro and in vivo. This study show that we may found a novel compound isolated from traditional Chinese medicines with potent anti-HBV function.