Elucidating the neuropathophysiology of COVID-19 using quantum dot biomimetics of SARS-CoV-2
Elucidating the neuropathophysiology of COVID-19 using quantum dot biomimetics of SARS-CoV-2
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DOI：
10.1117/12.2609118
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发表时间：
2022-03
期刊：
影响因子：
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通讯作者：
W. Chiang;J. Urban;Angela Litzburg;B. Nilsson;H. Gelbard;Todd D. Krauss
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作者：
W. Chiang;J. Urban;Angela Litzburg;B. Nilsson;H. Gelbard;Todd D. Krauss
将量子点封装在聚合物磷脂胶束中，这些磷脂胶束与SARS-COV-2尖峰蛋白的多个配体共轭，以形成用于SARS-COV-2（COVID-QDS）的荧光仿生纳米颗粒。将磷脂酰乙醇胺聚丙烯乙二醇（PE：PEG）添加给BIS（4-甲基苯基）磺极，形成PE：PEG：PEG：BIS-硫酮：Bis-硫酮，并通过细纤维重新汇集来形成CDSE/CDS CORE/SHELL量子量表周围的胶束。引入双隆组的胶束封装量子点的表面为通过双烷基化机制提供了多个位点，以与His标记的SARS-COV-2尖峰蛋白结合。基于洗脱的未结合的分数，我们估计平均每种covid-qD共轭七个尖峰蛋白。我们用这些共同QD构建体处理了神经血管单元（NVU）的体外模型系统，以研究COVID-QD，并且通过代理SARS-COV-2，可以调节NVU，从而导致COVID-19S相关的神经病理生理学。
Quantum dots were encapsulated in polymeric phospholipid micelles conjugated to multiple ligands of SARS-CoV-2 spike protein to form fluorescent biomimetic nanoparticles for SARS-CoV-2 (COVID-QDs). Phosphatidylethanolaminepolyethylene glycol (PE:PEG) was appended with bis(4-methylphenyl)sulfone to form PE:PEG:bis-sulfone and selfassembled into micelles around CdSe/CdS core/shell quantum dots via thin-film rehydration. The introduction of the bissulfone group the surface of the micelle-encapsulated quantum dots provides multiple sites for conjugation to his-tagged SARS-CoV-2 spike protein via a bisalkylation mechanism. Based on the eluted unconjugated fraction, we estimate that an average of seven spike proteins are conjugated per COVID-QD. We treated an in-vitro model system for the neurovascular unit (NVU) with these COVID-QD constructs to investigate the COVID-QDs, and by proxy SARS-CoV-2, may modulate the NVU leading to the COVID-19 associated neuropathophysiology.