Nonylphenol polyethoxylates degraded by three different wavelengths of UV and their genotoxic change-detected by generation of γ-H2AX.
Nonylphenol polyethoxylates degraded by three different wavelengths of UV and their genotoxic change-detected by generation of γ-H2AX.
复制标题
壬基酚聚乙氧基化物被三种不同波长的紫外线降解，并通过生成 γ-H2AX 检测其基因毒性变化。
DOI：
10.1111/php.12002
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发表时间：
2013
影响因子：
3.3
通讯作者：
T. Toyooka and Y. Ibuki
中科院分区：
文献类型：
--
作者：
T.Kubota;T. Toyooka and Y. Ibuki
阳光中的紫外线是化学品降解的重要因素。在这项研究中，我们研究了使用 UVA、B 和 C 来降解非离子表面活性剂、具有 10 或 70 个环氧乙烷 (EO) 单元的壬基酚聚乙氧基化物 (NPEO)，以及它们基于组蛋白 H2AX (γ-H2AX)（DNA 损伤标记物）磷酸化的遗传毒性变化。 NPEOs的降解取决于UV的能量，也就是说，具有最高能量的UVC是最有效的，而具有最低能量的UVA几乎没有引起任何变化。 NPEO(70) 的 EO 侧链在苯环附近被 UV 断裂，产生 EO 链缩短（约 10 个单元）的 NPEO。 γ-H2AX的产生反映了降解的模式； EO链的缩短将NPEO(70)转变为γ-H2AX的诱导剂，并且NPEO(10)的降解减弱了遗传毒性。 NPEO(10) 和紫外线降解的 NPEO(70) 产生的 γ-H2AX 与细胞周期无关。凝胶电泳检测到DNA双链断裂的形成与γ-H2AX的结果一致。这些结果表明，紫外线可以根据 EO 侧链的降解使 NPEO 无害或具有基因毒性，其效果取决于波长。
UV rays in sunlight are an important factor in the degradation of chemicals. In this study, we investigated the degradation of nonionic surfactants, nonylphenol polyethoxylates (NPEOs) with 10 or 70 ethylene oxide (EO) units using UVA, B and C, and their genotoxic change based on phosphorylation of histone H2AX (γ‐H2AX), a marker of DNA damage. NPEOs were degraded dependent on the energy of UV, that is, UVC having the highest energy was most effective, whereas UVA having the lowest energy caused little change. The EO side chain of NPEO(70) was broken near the benzene ring by UV, producing NPEOs with a shortened EO chain (around 10 units). The generation of γ‐H2AX reflected the pattern of degradation; shortening of the EO chain changed NPEO(70) into an inducer for γ‐H2AX, and degradation of NPEO(10) attenuated the genotoxicity. The γ‐H2AX generated by NPEO(10) and UV‐degraded NPEO(70) was independent of the cell cycle. The formation of DNA double strand breaks detected by gel electrophoresis was consistent with the results for γ‐H2AX. These results suggested that UV rays can make NPEOs harmless or genotoxic according to the degradation of the EO side chain, the effects being dependent on wavelength.