Detection of zearalenone and its metabolites in naturally contaminated porcine follicular fluid by using liquid chromatography-tandem mass spectrometry.
Detection of zearalenone and its metabolites in naturally contaminated porcine follicular fluid by using liquid chromatography-tandem mass spectrometry.
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DOI：
10.1262/jrd.10-106m
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发表时间：
2011-04
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通讯作者：
R. Sambuu;M. Takagi;S. Shiga;S. Uno;E. Kokushi;Z. Namula;T. Otoi;A. Miyamoto;E. Deguchi;J. Fink-Gremmels
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作者：
R. Sambuu;M. Takagi;S. Shiga;S. Uno;E. Kokushi;Z. Namula;T. Otoi;A. Miyamoto;E. Deguchi;J. Fink-Gremmels
玉米赤霉烯酮（ZEN）及其代谢产物是重要的非甾体类雌激素类真菌毒素，可引起家畜，尤其是猪的生殖障碍。采用液相色谱-串联质谱法同时检测猪卵泡液（FF）中ZEN及其代谢产物α-玉米赤霉烯醇（α-ZOL）和β-玉米赤霉烯醇（β-ZOL）。在从10个卵巢内共存卵泡（直径≥ 6 mm）采集的所有合并FF样本中均检出ZEN和α-ZOL，但未检出β-ZOL。此外，在用β-葡萄糖醛酸苷酶/芳基硫酸酯酶预处理的样品中检测到ZEN和α-ZOL，但在未处理的样品中未检测到，表明FF样品含有葡萄糖醛酸结合形式的真菌毒素，由于葡萄糖醛酸化影响受体结合，因此对猪卵母细胞的危害可能较小。尽管如此，应在体外和体内研究葡萄糖醛酸苷结合形式的作用。
Zearalenone (ZEN) and its metabolites are important nonsteroidal estrogenic mycotoxins that cause reproductive disorders in domestic animals, especially pigs. We aimed to simultaneously detect ZEN and its metabolites á-zearalenol (α-ZOL) and β-zearalenol (β-ZOL) in porcine follicular fluid (FF) by liquid chromatography-tandem mass spectrometry. ZEN and α-ZOL, but not β-ZOL, were detected in all pooled FF samples collected from coexisting follicles (diameter ≥ 6 mm) within 10 ovaries. Furthermore, ZEN and α-ZOL were detected in samples pretreated with β-glucuronidase/arylsulfatase, but not in those left untreated, suggesting that the FF samples contained glucuronide-conjugated forms of the mycotoxins that may be less harmful to porcine oocytes due to glucuronidation affecting the receptor binding. Nonetheless, the effects of the glucuronide-conjugated forms should be studied, both in vitro and in vivo.