Eudesmanolides from Wedelia trilobata (L.) Hitchc. as Potential Inducers of Plant Systemic Acquired Resistance
Eudesmanolides from Wedelia trilobata (L.) Hitchc. as Potential Inducers of Plant Systemic Acquired Resistance
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来自三叶蟛蜞菊 (Wedelia trilobata (L.) Hitchc) 的 Eudesmanolides。
DOI：
10.1021/jf400390e
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发表时间：
2013-04-24
影响因子：
6.1
通讯作者：
Li, Shunlin
中科院分区：
文献类型：
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作者：
Li, Yating;Hao, Xiaojiang;Li, Shunlin
从三裂叶婆婆纳（Wedelia trilobata（L.）希契奇，一种在中国南方臭名昭著的入侵杂草作为W.用常规的半叶法和叶盘法沿着以Westernblot法测定了8种桉烷内酯类化合物的抗烟草花叶病毒（TMV）活性。在10 μ g/mL浓度下，所有化合物对烟草植株的抗病毒活性均较强，抑制率为46.7%~ 76.5%，显著高于阳性对照宁南霉素（13.5%）。对化合物1和8诱导系统获得抗性的潜力进行了评价，化合物1和8表现出优异的诱导活性。此外，还发现不同浓度的化合物1促进烟草植株中苯丙氨酸解氨酶（PAL）活性。据我们所知，这是首次报道桉烷内酯可以诱导烟草植物对病毒病原体TMV的抗性。
Ten eudesmanolides (1-10) including five new ones (1-4 and 6) were isolated from the whole plant of Wedelia trilobata (L.) Hitchc., a notoriously invasive weed in South China. As main constituents of W. trilobata, eight of these eudesmanolides were tested for their antitobacco mosaic virus (TMV) activities by the conventional half-leaf and leaf-disk method along with Western blot analysis. All of the tested compounds, at 10 mu g/mL, showed strong antiviral activities in the pretreated tobacco plants with inhibition rates ranging from 46.7% to 76.5%, significantly higher than that of the positive control, ningnanmycin (13.5%). Their potential of inducing systemic acquired resistance (SAR) was also evaluated, and compounds 1 and 8 showed excellent induction activities. Furthermore, it was found that different concentrations of compound 1 promoted phenylalanine ammonia-lyase (PAL) activity in tobacco plants. To our knowledge, this is the first report that eudesmanolides could induce resistance in tobacco plants against the viral pathogen TMV.