Elucidating the Antiviral Mechanism of Different MARCH Factors.
Elucidating the Antiviral Mechanism of Different MARCH Factors.
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DOI：
10.1128/mbio.03264-20
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发表时间：
2021-03-02
期刊：
影响因子：
6.4
通讯作者：
Stavrou S
中科院分区：
文献类型：
--
作者：
Umthong S;Lynch B;Timilsina U;Waxman B;Ivey EB;Stavrou S
本研究探讨了不同MARCH蛋白限制逆转录病毒感染的机制。MARCH蛋白阻断包膜糖蛋白与出芽病毒粒子的结合。膜相关RING-CH（MARCH）蛋白属于E3泛素连接酶家族，其主要功能是从质膜去除跨膜蛋白。最近的工作表明，人MARCH 1，2和8是抗逆转录病毒因子，通过减少其在出芽病毒体中的掺入而靶向人免疫缺陷病毒1型（HIV-1）包膜糖蛋白。然而，缺乏有关该蛋白家族的抗病毒机制的信息，需要进一步研究。在这项研究中，使用人类MARCH蛋白和它们的小鼠同源物，我们提供了一个全面的分析这个家族的蛋白质的抗逆转录病毒机制。此外，我们表明，人类MARCH蛋白限制不同程度的包膜糖蛋白的不同数量的病毒。本报告阐明了MARCH蛋白的重要抗病毒功能及其在细胞内在免疫中的意义。
This study examines the mechanism utilized by different MARCH proteins to restrict retrovirus infection. MARCH proteins block the incorporation of envelope glycoproteins to the budding virions. The membrane-associated RING-CH (MARCH) proteins belong to a family of E3 ubiquitin ligases, whose main function is to remove transmembrane proteins from the plasma membrane. Recent work has shown that the human MARCH1, 2, and 8 are antiretroviral factors that target the human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins by reducing their incorporation in the budding virions. Nevertheless, the dearth of information regarding the antiviral mechanism of this family of proteins necessitates further examination. In this study, using both the human MARCH proteins and their mouse homologues, we provide a comprehensive analysis of the antiretroviral mechanism of this family of proteins. Moreover, we show that human MARCH proteins restrict to various degrees the envelope glycoproteins of a diverse number of viruses. This report sheds light on the important antiviral function of MARCH proteins and their significance in cell intrinsic immunity.