Role of nitric oxide pathway in placental dysfunction following fetal bypass
Role of nitric oxide pathway in placental dysfunction following fetal bypass
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DOI：
10.1016/j.athoracsur.2007.04.057
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发表时间：
2007-09-01
影响因子：
4.6
通讯作者：
Eghtesady, Pirooz
中科院分区：
文献类型：
--
作者：
Lam, Christopher;Baker, Scott;Eghtesady, Pirooz
背景胎儿体外循环术后胎盘功能障碍的病因尚不清楚。胎盘一氧化氮（NO）途径已牵连在这种病理生理。我们着手研究可能的扰动，在这一途径中的羊模型胎儿旁路。将妊娠100至114天的绵羊胎儿（n = 14）放置在旁路上30分钟，并在旁路后2小时进行测量血液动力学和脐带血流量。假手术对照组（n = 6）进行了器械植入，但未进行旁路手术。实时，体内NO浓度测定胎盘循环。为了检查NO途径的其他组分，通过免疫测定法分析胎儿血浆样品的总NO代谢物和环鸟苷3 '，5'-环一磷酸（cGMP）水平。同时用免疫组化法检测胎盘组织中磷酸二酯酶-5的表达。采用方差分析和最小显著性差异事后检验进行统计学分析（p
Background. The etiology of placental dysfunction after fetal cardiopulmonary bypass remains unknown. The placental nitric oxide ( NO) pathway has been implicated in this pathophysiology. We set out to examine possible perturbations in this pathway in an ovine model of fetal bypass.Methods. Ovine fetuses ( n = 14) between 100 and 114 days of gestation, instrumented to measure hemodynamics and umbilical blood flow, were placed on bypass for 30 minutes and followed after bypass for 2 hours. Sham controls ( n = 6) were instrumented but did not undergo bypass. Real-time, in-vivo NO concentrations were measured in the placental circulation. To examine other components of the NO pathway, fetal plasma samples were analyzed by immunoassays for total NO metabolite and cyclic guanosine 3', 5'-cyclic monophosphate ( cGMP) levels. In addition, the expression of phosphodiesterase-5 was examined in placenta by immunohistochemistry. Statistical analysis was performed using analysis of variance with least significant difference post hoc tests ( p