Fast optically sectioned fluorescence HiLo endomicroscopy
Fast optically sectioned fluorescence HiLo endomicroscopy
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DOI：
10.1117/1.jbo.17.2.021105
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发表时间：
2012-02-01
影响因子：
3.5
通讯作者：
Mertz, Jerome
中科院分区：
文献类型：
--
作者：
Ford, Tim N.;Lim, Daryl;Mertz, Jerome
我们描述了一种非扫描，光纤束显微内镜，进行光学切片荧光成像与快速帧速率和实时处理。我们的切片技术是基于HiLo成像，其中两个宽视场图像下获得均匀和结构化的照明和数值处理，以拒绝离焦的背景。这项工作是一个改进后，较早的演示宽视场光学切片通过一个灵活的光纤束。改进后的设备具有横向和轴向分辨率分别为2.6和17 μ m，9.5 Hz的净帧速率通过实时图像处理与图形处理单元（GPU）和显着减少的运动伪影通过使用双快门相机获得。我们展示了我们的系统的性能与光学切片的图像和视频的荧光标记的绒毛尿囊膜（CAM）在发展G。鸡胚HiLo显微内镜是低成本、高速临床光学活检的候选技术。(C)2012年，美国光电仪器工程师学会（SPIE）。[DOI：10.1117/1.JBO.17.2.021105]
We describe a nonscanning, fiber bundle endomicroscope that performs optically sectioned fluorescence imaging with fast frame rates and real-time processing. Our sectioning technique is based on HiLo imaging, wherein two widefield images are acquired under uniform and structured illumination and numerically processed to reject out-of-focus background. This work is an improvement upon an earlier demonstration of widefield optical sectioning through a flexible fiber bundle. The improved device features lateral and axial resolutions of 2.6 and 17 mu m, respectively, a net frame rate of 9.5 Hz obtained by real-time image processing with a graphics processing unit (GPU) and significantly reduced motion artifacts obtained by the use of a double-shutter camera. We demonstrate the performance of our system with optically sectioned images and videos of a fluorescently labeled chorioallantoic membrane (CAM) in the developing G. gallus embryo. HiLo endomicroscopy is a candidate technique for low-cost, high-speed clinical optical biopsies. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.2.021105]