Cadmium-induced ultramorphological and physiological changes in leaves of two transgenic cotton cultivars and their wild relative
Cadmium-induced ultramorphological and physiological changes in leaves of two transgenic cotton cultivars and their wild relative
复制标题
镉诱导的两个转基因棉花品种及其野生近缘种叶片的超形态和生理变化
DOI：
10.1016/j.jhazmat.2009.02.069
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发表时间：
2009-09-15
影响因子：
13.6
通讯作者：
Zhu, Shuijin
中科院分区：
文献类型：
--
作者：
Daud, M. K.;Variath, M. T.;Zhu, Shuijin
本研究利用超微学和生理指标对两个转基因棉花品种（BR001和GK30）及其野生近缘品种（Coker 312）叶片和全株镉诱导的变化进行了研究。随着Cd浓度的升高（即10、100、1000亩），根、茎、叶的平均长度、叶宽及其鲜、干生物量均比对照呈线性下降。根、茎、叶的吸水能力逐渐增强，叶片吸水能力最强，其次是根和茎。BR001累积镉最多，其次是GK30和Coker 312。根和茎对镉的吸收与叶片、茎和根的吸水量呈极显著的直接相关。镉胁迫下叶片叶肉细胞超微结构发生改变。其中以BROW最为明显，其次为GK30和Coker 312。Cd对光合器官的定性影响主要表现在叶绿体形态的改变、淀粉粒数量和大小的增加以及质体舌的增加。Cd以电子致密颗粒的形式存在于液泡内，并附着在细胞壁上。从目前的实验中，可以很好地确定，这些品种的Cd脱毒都参与了外胞体和共塑结合。没有胞浆内陷表明Cd毒性水平不会引起任何品种的水分胁迫。此外，这些品种对Cd的积累和吸收能力也存在差异。(c) 2009 Elsevier B.V.版权所有
The present study describes cadmium-induced alterations in the leaves as well as at the whole plant level in two transgenic cotton cultivars (BR001 and GK30) and their wild relative (Coker 312) using both ultramorphological and physiological indices. With elevated levels of Cd (i.e. 10, 100, 1000 mu M), the mean lengths of root, stem and leaf and leaf width as well as their fresh and dry biomasses linearly decreased over their respective controls. Moreover, root, stem and leaf water absorption capacities progressively stimulated, which were high in leaves followed by roots and sterns. BR001 accumulated more cadmium followed by GK30 and Coker 312. Root and shoot cadmium uptakes were significantly and directly correlated with each other as well as with leaf, stem and root water absorption capacities. The ultrastructural modifications in leaf mesophyll cells were triggered with increase in Cd stress regime. They were more obvious in BROW followed by GK30 and Coker 312. Changes in morphology of chloroplast, increase in number and size of starch grains as well as increase in number of plastoglobuli were the noticed qualitative effects of Cd on photosynthetic organ. Cd in the form of electron dense granules could be seen inside the vacuoles and attached to the cell walls in all these cultivars. From the present experiment, it can be well established that both apoplastic and symplastic bindings are involved in Cd detoxification in these cultivars. Absence of tonoplast invagination reveals that Cd toxic levels did not cause water stress in any cultivars. Additionally, these cultivars possess differential capabilities towards Cd accumulation and its sequestration. (c) 2009 Elsevier B.V. All rights reserved.