An HPLC method for determination of oridonin in rabbits using isopsoralen as an internal standard and its application to pharmacokinetic studies for oridonin-loaded nanoparticles
An HPLC method for determination of oridonin in rabbits using isopsoralen as an internal standard and its application to pharmacokinetic studies for oridonin-loaded nanoparticles
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DOI：
10.1016/j.jchromb.2008.05.005
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发表时间：
2008-06-15
影响因子：
3
通讯作者：
Wei, Li
中科院分区：
文献类型：
--
作者：
Mei, Yinghao;Xu, Jie;Wei, Li
建立了测定家兔血浆中冬凌草甲素浓度的高效液相色谱法。采用简单的液-液萃取（LLE）方法从家兔血浆中提取ORI和内标物异补骨脂素（IS）。采用Kronnasil C18 5 μ m色谱柱（250 mm x 4.6 mm），以甲醇-水（50：50，v/v）为移动的相，流速为1 mL/min，紫外检测波长为241 nm。定量下限（LLOQ）为0.02 μ g/mL。校准曲线在0.02-10 μ g/mL的浓度范围内呈线性。含量测定准确度和精密度分别在95.1-113.5%和5.4- 8.6%范围内。该HPLC方法成功地应用于ORI负载的聚（己内酯）-聚（环氧乙烷）-聚（己内酯）共聚物纳米颗粒（ORI-PCL-PEO-PCL-NP）在兔体内的药代动力学研究，以相当于2 mg ORI/kg的剂量单次静脉注射给药，并将ORI的药代动力学参数与单次静脉注射相同剂量的ORI溶液进行比较。(c)2008 Elsevier B. V.保留所有权利。
A simple and sensitive HPLC method has been developed and validated for the determination of oridonin (ORI) in rabbit plasma. A simple liquid-liquid extraction (LLE) method was applied to extract ORI and the internal standard (IS), isopsoralen, from rabbit plasma. Chromatographic separation of ORI and the IS was achieved with a Kronnasil C18 5-mu m column (250 mm x 4.6 mm) using methanol-water (50:50, v/v) as mobile phase at a flow rate of 1 mL/min. The ultraviolet (UV) detection wavelength was set at 241 nm. The lower limit of quantification (LLOQ) was 0.02 mu g/mL. The calibration curves were linear over a concentration range of 0.02-10 mu g/mL. The assay accuracy and precision were within the range of 95.1-113.5% and 5.4-8.6%, respectively. This HPLC method was applied successfully to the pharmacokinetic study of ORI-loaded poly(caprolactone)-poly(ethylene oxide)-poly(caprolactone) copolymer nanoparticles (ORI-PCL-PEO-PCL-NP) in rabbits, given as a single intravenous injection at the dose equivalent to 2 mg of ORI/kg, and the pharmacokinetic parameters for ORI were compared with a single intravenous injection of a ORI solution at the same dose. (c) 2008 Elsevier B.V. All rights reserved.