Selectively Imaging Single Gold Nanorods by Polarized Light Microscopy with Low Background
Selectively Imaging Single Gold Nanorods by Polarized Light Microscopy with Low Background
复制标题
通过低背景偏光显微镜选择性成像单金纳米棒
DOI：
10.1007/s11468-015-0011-6
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发表时间：
2015
期刊：
影响因子：
3
通讯作者：
Xu Keqian
中科院分区：
文献类型：
--
作者：
Chen Yiping;Chen Xuechu;Cao Qian;Xu Keqian
基于单金纳米棒的光去偏振光特性，提出了一种利用偏振光显微镜成像单金纳米棒的新方法。结果表明，在PLM下可以选择性地观察到gnr，而球形金纳米颗粒只能在暗场显微镜下观察到。PLM应用于单纳米颗粒成像有许多突出的特点。例如，gnr的平均光斑面积为71±26像素，仅为DFM下的42%。背景明显降低，信噪比提高至少3.6倍。即使在极低背景下，也能清晰地检测到细胞内的gnr，并且发现细胞内gnr的分布是不均匀的。gnr与PLM结合可以在生物医学和临床应用以及基础研究中找到各种用途。
We introduce a novel method for single gold nanorods (GNRs) imaging by polarized light microscopy (PLM) based on their light depolarization light property. The results demonstrate that GNRs can be selectively seen by PLM, while sphere gold nanoparticles only can be observed under darkfield microscopy (DFM). When PLM is applied in single nanoparticle imaging, there are many outstanding features. For example, the average light spot of GNRs occupies 71 ± 26 pixels, which is only 42 % of that under DFM. The background is significantly reduced and signal-to-noise ratio is greatly improved by at least 3.6 times. Even GNRs in cells can be clearly detected with extreme low background, and the distributions of GNRs in the intracellular space were found to be ununiform. GNRs combined with PLM may find various uses in biomedical and clinical applications as well as in fundamental studies.