Characterization of the 1918 "Spanish" influenza virus neuraminidase gene
Characterization of the 1918 "Spanish" influenza virus neuraminidase gene
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DOI：
10.1073/pnas.100140097
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发表时间：
2000-06-06
影响因子：
11.1
通讯作者：
Taubenberger, JK
中科院分区：
文献类型：
--
作者：
Reid, AH;Fanning, TG;Taubenberger, JK
1918年“西班牙”流感大流行的特点是死亡率特别高，特别是在年轻人中。流感病毒的表面蛋白，血凝素和神经氨酸酶，在毒力、宿主特异性和人体免疫应答中起重要作用。去年报道了血凝素的完整编码序列。这个实验室现在已经确定了1918年病毒的神经氨酸酶基因的完整编码序列。从1918年流感的三名受害者的肺组织中分离出流感病毒RNA片段;从A/Brevig使命/1/18中生成完整序列，并对A/南卡罗来纳州/1/18和A/纽约/1/18进行确认性测序。将1918株神经氨酸酶基因序列与其他N1亚型神经氨酸酶基因进行比较，包括本研究新测序的9株N1菌株。1918神经氨酸酶与禽类菌株共享许多序列和结构特征，包括保守的活性位点、野生型茎长度、糖基化位点和抗原位点。从系统发生学上看，1918年的神经氨酸酶基因似乎介于哺乳动物和鸟类之间，这表明它是在1918年大流行之前引入哺乳动物的。
The "Spanish" influenza pandemic of 1918 was characterized by exceptionally high mortality, especially among young adults. The surface proteins of influenza viruses, hemagglutinin and neuraminidase, play important roles in virulence, host specificity, and the human immune response. The complete coding sequence of hemagglutinin was reported last year. This laboratory has now determined the complete coding sequence of the neuraminidase gene of the 1918 virus. Influenza RNA fragments were isolated from lung tissue of three victims of the 1918 flu; complete sequence was generated from A/Brevig Mission/1/18, with confirmatory sequencing carried out on A/South Carolina/1/18 and A/New York/1/18. The 1918 neuraminidase gene sequence was compared with other N1 subtype neuraminidase genes, including 9 N1 strains newly sequenced for this study. The 1918 neuraminidase shares many sequence and structural characteristics with avian strains, including the conserved active site, wild-type stalk length, glycosylation sites, and antigenic sites. Phylogenetically, the 1918 neuraminidase gene appears to be intermediate between mammals and birds, suggesting that it was introduced into mammals just before the 1918 pandemic.