The elimination and duplication of lower part of blastoderm effects on the number of primordial germ cells in goldfish
The elimination and duplication of lower part of blastoderm effects on the number of primordial germ cells in goldfish
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金鱼下胚层消除和复制对原始生殖细胞数量的影响
DOI：
10.2331/fishsci.65.577
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发表时间：
1999
影响因子：
1.9
通讯作者：
F. Yamazaki
中科院分区：
文献类型：
--
作者：
M. Kazama;E. Yamaha;F. Yamazaki
通过对金鱼胚胎发育过程中原始生殖细胞(PGCs)的组织学观察和胚泡发育中期胚胎层的部分消除和复制对原始生殖细胞数量的影响，研究了原始生殖细胞的起源和移位。金鱼原生殖细胞与体细胞的区别在于其体积大、轮廓清晰、核大，因为它们在形态上与其他鱼种的相同。它们在受精后10天定位于性腺原基，并可追溯到受精后30小时的胚胎，具有7~9个体节。在30hpf的胚胎中，除脑、脊髓、脊索和包膜层外，其他部位均有分布。从受精后30h到10d，原生殖细胞的数量没有增加。当胚泡下部在囊胚期中期消失时，性腺原基的原生殖细胞数量减少。但是，去除同一时期的每部分UP并不影响原生粒细胞的数量。此外，在将正常胚泡移植到去除胚泡上部的宿主胚胎上的重复胚胎中，PGCs的数量增加。这些结果表明，在胚泡中期，可由细胞质因子预先决定的PGCs细胞已分布于胚泡下部，之后不能再生。
The origin and translocation of primordial germ cells (PGCs) in goldfish Carassius auratus were studied, using both histological observations during embryonic development and by following the effects of partial elimination and duplication of blastoderm on the numbers of PGCs at the mid-blastu la stage. Goldfish PGCs are distinguished from somatic cells by their large size, distinct outline and large nucleus, because they are morphologically identical with those of the other fish species . They were locat ed in the gonadal anlage at ten days post-fertilization, and were traced back to 30-hour post-fertiliza tion (hpf) embryos with seven to nine somites. They were distributed widely in an embryo at 30 hpf, ex cept for brain, spinal cord, notochord and the enveloping layer. The numbers of PGCs did not increase from 30 hours to ten days post-fertilization. When the lower part of the blastoderm was eliminated at the mid-blastula stage, the number of PGCs decreased at the gonadal anlage. But, elimination of the up per part at same stage did not effect the number of PGCs. Moreover, the number of PGCs increased in the duplicated embryos, in which a normal blastoderm was transplanted onto a host embryo from which the upper part of the blastoderm had been removed. These results suggest that the PGCs-bear ing-cells could be predetermined by cytoplasmic factor and were already distributed in the lower part of blastoderm at the mid-blastula stage, and that they can not regenerate after that.
影响因子：
4.6
作者：
D. Kane;C. Kimmel
通讯作者：
D. Kane;C. Kimmel
影响因子：
4.6
作者：
C. Kimmel;R. M. Warga;T. Schilling
通讯作者：
C. Kimmel;R. M. Warga;T. Schilling