Developing an analytical method for free amino acids in atmospheric precipitation using gas chromatography coupled with mass spectrometry
Developing an analytical method for free amino acids in atmospheric precipitation using gas chromatography coupled with mass spectrometry
复制标题
使用气相色谱-质谱法开发大气降水中游离氨基酸的分析方法
DOI：
10.1016/j.atmosres.2021.105579
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发表时间：
2021-03-21
影响因子：
5.5
通讯作者：
Cong, Zhiyuan
中科院分区：
文献类型：
--
作者：
Gao, Shaopeng;Xu, Baiqing;Cong, Zhiyuan
雨样品中的氨基酸为研究其来源及其在生物地球化学循环中的作用提供了重要信息。本文介绍了气相色谱-质谱联用（GC - MS）分析大气降水中氨基酸的一种新方法的开发和验证。该方法的检出限（MDLs）为1.46 ng L-1（脯氨酸）~ 135 ng L-1（精氨酸），远低于高效液相色谱（HPLC）等方法，与液相色谱-串联质谱（LC-MS/MS）相媲美。空白样品中没有检测到目标分析物，这是由于我们采用了优化的预处理方案。我们的结论是GC？本研究开发的基于质谱的技术具有检出限低的优势。在TP雨、海洋雨和北极冰中发现了相似的氨基酸相对丰度。鉴于雪和雨样品的相似性，我们的方法也为冰川冰芯中氨基酸的未来分析提供了潜力。
Amino acids in rain samples offer important information on their origin and roles in biogeochemical cycling. This paper describes the development and validation of an alternative analytical procedure for amino acids in atmospheric precipitation using gas chromatography coupled with mass spectrometry (GC?MS). The method detection limits (MDLs) of these amino acids ranged from 1.46 ng L-1 (Proline) to 135 ng L-1 (Arginine), which were much lower than previous methods like high-performance liquid chromatography (HPLC) and comparable to those with liquid chromatography-tandem mass spectrometry (LC-MS/MS). None of the target analytes were detected in blank samples, which was attributed to the optimized pretreatment protocol we adopted. We conclude that the GC?MS based technique developed in this study possesses advantage due to low detection limits. We further demonstrate the applicability of this method by analyzing rain samples from the background atmosphere of the Tibetan Plateau (TP). Similar relative abundances of amino acids were found in the TP rains, marine rains and Arctic ice. The correlations between FAAs and water- soluble ions in the Tibetan Plateau rainwaters showed that the FAAs were most likely originated from biomass burning. Given the similarity of snow and rain samples, our method also provides potential for the future analysis of amino acids in glacier ice cores.