Adverse effects of coumestrol and genistein on mammary morphogenesis and future milk production ability of mammary epithelial cells.
Adverse effects of coumestrol and genistein on mammary morphogenesis and future milk production ability of mammary epithelial cells.
复制标题
香豆雌酚和金雀异黄酮对乳腺形态发生和乳腺上皮细胞未来产奶能力的不利影响。
DOI：
10.1002/adbi.201900187
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发表时间：
2020
影响因子：
4.1
通讯作者：
Kobayashi K
中科院分区：
文献类型：
--
作者：
Kumai A;Tsugami Y;Suzuki N;Suzuki T;Nishimura T;Kobayashi K
异黄酮是豆类中存在的一类黄酮类化合物，因其具有类似雌激素的活性而被称为植物雌激素。众所周知，内源性雌激素可以调节怀孕期间的乳腺形态发生。每种异黄酮还具有不同的生理活性。然而，由于异黄酮被肠道细菌代谢成不同的异黄酮，因此很难在体内研究每种异黄酮对乳腺形态发生的直接影响。在这项研究中，使用体外培养模型研究了香豆雌酚、大豆黄酮和金雀异黄酮对乳腺结构发育和乳腺上皮细胞（MEC）未来产奶能力的直接影响。将小鼠 MEC 在含有碱性成纤维细胞生长因子和表皮生长因子的基质胶中培养，分别诱导导管分支和肺泡形成。 Coumestrol 和金雀异黄素通过影响 MEC 的增殖和迁移并诱导细胞凋亡来抑制导管分支和肺泡形成。大豆苷元几乎不影响乳腺结构的发育。此外，香豆雌酚预处理会对 MEC 的产奶能力产生不利影响。这些结果表明，每种异黄酮通过影响 MEC 行为，对乳腺形态发生和未来产奶量产生不同的影响。这些结果还表明培养模型可以有效地研究体外乳腺上皮形态发生。
Isoflavones are a class of flavonoids present in legumes and are called phytoestrogens because of their estrogen‐like activity. Endogenous estrogen is well known to regulate mammary gland morphogenesis during pregnancy. Each isoflavone also has different physiological activities. However, it is difficult to investigate the direct effect of each isoflavone in mammary morphogenesis in vivo because isoflavones are metabolized into different isoflavones by enteric bacteria. In this study, investigated are the direct influences of coumestrol, daidzein, and genistein on mammary structure development and future milk production ability of mammary epithelial cells (MECs) using in vitro culture models. Mouse MECs are cultured in Matrigel with basic fibroblast growth factor and epidermal growth factor to induce ductal branching and alveolar formation, respectively. Coumestrol and genistein inhibit ductal branching and alveolar formation by affecting the proliferation and migration of MECs with the induction of apoptosis. Daidzein hardly influences mammary structure development. Furthermore, pretreatment with coumestrol adversely affects the induction of milk production ability of MECs. These results suggest that each isoflavone differentially influences mammary morphogenesis and future milk production by affecting MEC behaviors. These results also suggest that the culture models are effective to study mammary epithelial morphogenesis in vitro.