Different curcuminoids inhibit T-lymphocyte proliferation independently of their radical scavenging activities
Different curcuminoids inhibit T-lymphocyte proliferation independently of their radical scavenging activities
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DOI：
10.1007/s11095-008-9579-2
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发表时间：
2008-08-01
影响因子：
3.7
通讯作者：
Kaever, Volkhard
中科院分区：
文献类型：
--
作者：
Deters, Michael;Knochenwefel, Heiko;Kaever, Volkhard
目的.我们研究了姜黄素及其衍生物和降解产物对OKT 3诱导的人外周血单个核细胞（PBMC）增殖的抑制作用及其自由基清除活性的作用。通过测量（3）H-胸苷掺入来确定OKT 3诱导的人PBMC增殖。采用DPPH自由基自由基清除活性测定法评价其清除自由基的活性。姜黄素、异姜黄素、双去甲氧基-、二乙酰-、四氢-、六氢-和八氢姜黄素以及香草醛、阿魏酸和二氢阿魏酸抑制OKT 3诱导的PBMC增殖，IC（50）值分别为2.8、2.8、6.4、1.0、25、38、82、729、457和> 1，000 μ M。自由基清除效果最强的调查物质是四氢，六氢，octahydrocurcumin与IC（50）值为10.0，11.7，和12.3 μ M，分别。二氢阿魏酸、阿魏酸和姜黄素的IC（50）值分别为19.5、37和40 μ M。清除自由基活性最低的物质是香草醛、异姜黄素、二乙酰姜黄素和双脱甲氧基姜黄素，IC（50）值均大于100 μ M。类姜黄素诱导的OKT 3诱导的PBMC增殖的抑制依赖于1，6-庚-3，5-二酮结构的碳原子数和双键数以及类姜黄素的酚环取代物，但与其各自的自由基清除活性无关。
Purpose. We investigated the inhibitory effects of curcumin, curcumin derivatives and degradation products on OKT3-induced human peripheral blood mononuclear cell (PBMC) proliferation and the role of their radical scavenging activity.Methods. OKT3-induced human PBMC proliferation was determined by measuring (3)H-thymidine incorporation. Radical scavenging activity was evaluated by using an in vitro DPPH assay.Results. OKT3-induced PBMC proliferation was inhibited by curcumin, isocurcumin, bisdesmethoxy-, diacetyl-, tetrahydro-, hexahydro-, and octahydrocurcumin as well as by vanillin, ferulic acid, and dihydroferulic acid with IC(50)-values of 2.8, 2.8, 6.4, 1.0, 25, 38, 82, 729, 457, and > 1,000 mu M, respectively. The investigated substances with the strongest effect on radical scavenging were tetrahydro-, hexahydro-, and octahydrocurcumin with IC(50) values of 10.0, 11.7, and 12.3 mu M, respectively. IC(50)-values of dihydroferulic acid, ferulic acid, and curcumin were 19.5, 37, and 40 mu M. The substances with the lowest radical scavenging activities were vanillin, isocurcumin, diacetylcurcumin, and bisdesmethoxycurcumin with IC(50) values higher than 100 mu M each.Conclusions. Curcuminoid-induced inhibition of OKT3-induced PBMC proliferation depends on the number of carbon atoms and double bonds of the 1,6-heptadiene-3,5-dione structure as well as on the phenolic ring substitutes of the curcuminoids but is not correlated to their respective radical scavenging activity.