Bone marrow cells differentiate into wound myofibroblasts and accelerate the healing of wounds with exposed bones when combined with an occlusive dressing
Bone marrow cells differentiate into wound myofibroblasts and accelerate the healing of wounds with exposed bones when combined with an occlusive dressing
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DOI：
10.1111/j.1365-2133.2005.06402.x
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发表时间：
2005-04-01
影响因子：
10.3
通讯作者：
Itami, S
中科院分区：
文献类型：
--
作者：
Yamaguchi, Y;Kubo, T;Itami, S
背景尽管越来越多的证据表明骨髓中含有间充质干细胞，这些细胞在进入特定组织微环境（小生境）后具有分化为各种类型细胞的多潜能性，但骨髓细胞在加速伤口愈合中的有用性尚未得到评估。活组织检查和免疫组织化学，当在大鼠伤口上局部施用时，来自绿色荧光蛋白（GFP）转基因大鼠的各种类型的细胞是否会分化成伤口组分细胞。我们还调查了局部应用的骨髓细胞与封闭敷料是否会加速愈合的伤口暴露的骨头，测量由planimetry.Results GFP标记的骨髓细胞含有多能干细胞，充分分化成伤口肌成纤维细胞与α-平滑肌肌动蛋白在肉芽组织。其他类型的细胞，包括肌细胞、脂肪细胞、血沉棕黄层的外周血细胞和真皮成纤维细胞，在形态学或组织化学上不表达肌成纤维细胞特征。应用骨髓细胞和封闭敷料加速修复的伤口暴露的骨头，封闭敷料只或局部施用骨髓细胞加半干dressing.Conclusions我们的研究表明，骨髓细胞加速伤口愈合至少部分通过其分化成伤口肌成纤维细胞。因此，用骨髓细胞和支持性封闭敷料治疗伤口可有效促进健康肉芽组织的形成，也可用于制备理想的伤口床。
Background The usefulness of bone marrow cells in accelerating wound healing has not been evaluated despite increasing evidence that bone marrow contains mesenchymal stem cells that have multipotentiality to differentiate into various types of cells after they enter the microenvironment of a specific tissue (niche).Objectives To determine the effects of bone marrow cells and occlusive dressings in promoting wound healing in rats.Methods We investigated by grafting, biopsy and immunohistochemistry whether various types of cells derived from green fluorescent protein (GFP)-transgenic rats would differentiate into wound component cells when administered topically on the wounds of rats. We also investigated whether topical application of bone marrow cells with an occlusive dressing would accelerate the healing of wounds with exposed bones, as measured by planimetry.Results GFP-labelled bone marrow cells contained multipotent stem cells that sufficiently differentiated into wound myofibroblasts presenting with alpha-smooth muscle actin in granulation tissue. Other types of cells, including myocytes, adipocytes, peripheral blood cells from buffy coat and dermal fibroblasts, did not express myofibroblast characteristics morphologically or immunohistochemically. Application of bone marrow cells and an occlusive dressing accelerated the repair of wounds with exposed bones, compared with an occlusive dressing only or with the topical administration of bone marrow cells plus a semidry to dry dressing.Conclusions Our study indicates that bone marrow cells accelerate the healing of wounds at least in part through their differentiation into wound myofibroblasts. Thus, treatment of wounds with bone marrow cells and a supportive occlusive dressing is effective in promoting the formation of healthy granulation tissue and also for the preparation of an ideal wound bed.