From molecules to mating: Rapid evolution and biochemical studies of reproductive proteins.
From molecules to mating: Rapid evolution and biochemical studies of reproductive proteins.
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DOI：
10.1016/j.jprot.2015.06.007
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发表时间：
2016-03-01
影响因子：
3.3
通讯作者：
Swanson WJ
中科院分区：
文献类型：
--
作者：
Wilburn DB;Swanson WJ
有性生殖和遗传信息的交换是生命之树所有分支的物种的基本生物过程。在过去的四十年里，生化学家们继续发现了许多促进生殖的因素，但介导这一过程的分子机制仍然让我们困惑。然而，在这项研究中，一个反复出现的观察结果是生殖蛋白的快速进化。在动物中，雄性和雌性的利益竞争常常导致相互作用的蛋白质对之间的军备竞赛。这一现象在生殖的所有阶段都可以观察到，包括信息素、精液成分和配子识别蛋白。在本文中，我们回顾了如何将进化理论与生物化学实验相结合来研究相互作用的生殖蛋白。例子包括从模式和非模式生物，并强调了最近的研究，因为他们使用了最先进的基因组和蛋白质组学技术。尽管经过了几十年的研究，我们对调节受精的分子机制的理解仍然很差。迄今为止，对模式生物和非模式生物的分子进化研究已经为阐明动物繁殖的分子基础提供了一些最好的推论。这篇综述文章详细介绍了40年来生化和进化实验如何共同促进了该领域的发展，以及最近使用高通量基因组学和蛋白质组学技术的工作如何为这一关键的生物过程提供了更多的见解。
Sexual reproduction and the exchange of genetic information are essential biological processes for species across all branches of the tree of life. Over the last four decades, biochemists have continued to identify many of the factors that facilitate reproduction, but the molecular mechanisms that mediate this process continue to elude us. However, a recurring observation in this research has been the rapid evolution of reproductive proteins. In animals, the competing interests of males and females often result in arms race dynamics between pairs of interacting proteins. This phenomenon has been observed in all stages of reproduction, including pheromones, seminal fluid components, and gamete recognition proteins. In this article, we review how the integration of evolutionary theory with biochemical experiments can be used to study interacting reproductive proteins. Examples are included from both model and non-model organisms, and recent studies are highlighted for their use of state-of-the-art genomic and proteomic techniques. Despite decades of research, our understanding of the molecular mechanisms that mediate fertilization remain poorly characterized. To date, molecular evolutionary studies on both model and non-model organisms have provided some of the best inferences to elucidating the molecular underpinnings of animal reproduction. This review article details how biochemical and evolutionary experiments have jointly enhanced the field for 40 years, and how recent work using high-throughput genomic and proteomic techniques have shed additional insights into this crucial biological process.