Prevalence and diversity of Arcobacter spp. isolated from the internal organs of spontaneous porcine abortions in Denmark
Prevalence and diversity of Arcobacter spp. isolated from the internal organs of spontaneous porcine abortions in Denmark
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DOI：
10.1016/s0378-1135(01)00503-x
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发表时间：
2002-03-01
影响因子：
3.3
通讯作者：
Vandamme, P
中科院分区：
文献类型：
--
作者：
On, SLW;Jensen, TK;Vandamme, P
为确定弯曲杆菌在丹麦猪流产中的流行率和可能的意义，从提交给丹麦兽医实验室的55头流产猪胎儿中采集了表面烧灼的肝脏和肾脏样品，并使用敏感的分离程序检查合并的组织样品中的弯曲杆菌、弓形杆菌和螺杆菌属。还进行常规微生物、免疫学和组织病理学检查，以确定并发感染或组织病理学变化。流产对确定的流产病原体（布鲁氏菌、钩端螺旋体、PPV和PRRSV）检测呈阴性，但从23/55例流产中回收了弓形杆菌属。在7/23例Arcostatin阳性胎儿和4/32例Arcostatin阴性胎儿中观察到猪链球菌、大肠杆菌和溶血性链球菌的合并感染。组织学分析确定研究组中有胎盘炎、肺炎、肝炎和脑炎。然而.没有明显的病理特征与Arcobacter阳性病例单独相关，在组织样品中也没有观察到Arcobacter样细菌。cryaerophilus和A.斯奇罗维。6个菌株不能归类到我的现有种属中，并且表型不同，因此，可能代表至少一个新种属。鉴定结果表明，在同一胎中可以发现多个分类群，在同一母猪的不同流产胎中也可以发现多个分类群。在丹麦猪流产中，Arcobacters的高流行率可能至少占未检测到既定流产剂的>90%的病例中的一部分，但需要进一步研究来确定每个物种的作用，特别是在与其他细菌共感染的情况下。(C)2002 Elsevier Science B. V.保留所有权利。
A study was conducted to determine the prevalence and possible significance of campylobacteria in pig abortions in Denmark, Surface-cauterised liver and kidney samples from 55 aborted pig fetuses submitted to the Danish Veterinary Laboratory were taken and a sensitive isolation procedure used to examine pooled tissue samples for Campylobacter, Arcobacter and Helicobacter spp. Routine microbiological, immunological, and histopathological examinations were also performed to identify concurrent infections or histopathological change. The abortions tested negative for established abortifacient pathogens (Brucella, Leptospira, PPV PRRSV), but Arcobacter spp, were recovered from 23/55 abortions. Co-infections with Streptococcus suis, Escherichia coli, and haemolytic streptococci were observed in 7/23 Arcobacter-positive fetuses, and in 4/32 Arcobacter-negative fetuses, Histopathological analyses identified placentitis, pneumonia, hepatitis and encephalitis among the study group. However. no obvious pathologic features were solely associated with Arcobacter-positive cases, nor were Arcobacter-like bacteria observed in tissue samples, Protein profile analyses of the 27 Arcobacter isolates identified I I as A. cryaerophilus and 10 as A. skirrowii. Six strains could not be classified into my existing species and were phenotypically distinct, thus, potentially representing at least one new species. The identification results showed that multiple taxa could be found in a single fetus, and in distinct aborted fetuses from a single sow. The high prevalence of arcobacters in Danish pig abortions may account for at least some of the >90% of cases in which no established abortifacient agent is detected, but further studies are needed to define the role of each species, especially where co-infections with other bacteria are present. (C) 2002 Elsevier Science B.V. All rights reserved.