Transcriptomic and virulence factors analyses of Cryptococcus neoformans hypoxia response
Transcriptomic and virulence factors analyses of Cryptococcus neoformans hypoxia response
复制标题
新型隐球菌缺氧反应的转录组和毒力因子分析
DOI：
10.1111/apm.12647
复制
发表时间：
2017-03-01
期刊：
影响因子：
2.8
通讯作者：
Sang, Hong
中科院分区：
文献类型：
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作者：
Kong, Qingtao;Yang, Rui;Sang, Hong
新生隐球菌是一种环境病原体，需要大气中的氧气才能最佳生长。吸入后，新生隐球菌会扩散到大脑，引起脑膜脑炎。然而，这种病原体适应大脑低氧环境的机制还没有被研究。我们从宿主组织中分离出一株带有小胶囊的新生葡萄球菌菌株，尽管该菌株在常氧条件下产生大胶囊。我们推测，包膜大小的这种差异归因于慢性炎症反应引起的低氧。本研究研究了低氧对新生葡萄球菌毒力因子(包括被膜、黑色素、尿素酶和磷脂酶)的影响，并对这些毒力相关基因进行了转录转录分析。我们发现新生葡萄球菌在低氧条件下生长，尽管生长缓慢，低氧可能抑制了新生葡萄球菌的被膜大小、黑色素产生以及磷脂酶和尿素酶的活性。
Cryptococcus neoformans is an environmental pathogen requiring atmospheric levels of oxygen for optimal growth. Upon inhalation, C. neoformans disseminates to the brain and causes meningoencephalitis. However, the mechanisms by which the pathogen adapts to the low‐oxygen environment in the brain have not been investigated. We isolated a C. neoformans strain with a small capsule from a host tissue, although this strain produces large capsules in normoxic conditions. We hypothesize that this difference in capsule size is attributed to hypoxia caused by chronic inflammatory response. This study investigated the effect of hypoxia on virulence factors (including capsule, melanin, urease, and phospholipase) of C. neoformans and conducted transcriptomic analyses of the virulence‐associated genes. We found that C. neoformans grew under hypoxic condition, albeit slowly, and that hypoxia may have inhibited the capsule size, melanin production, and phospholipase and urease activities in C. neoformans.