α-glucosidase inhibitors reduce dengue virus production by affecting the initial steps of virion morphogenesis in the endoplasmic reticulum
α-glucosidase inhibitors reduce dengue virus production by affecting the initial steps of virion morphogenesis in the endoplasmic reticulum
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DOI：
10.1128/jvi.74.1.564-572.2000
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发表时间：
2000-01-01
影响因子：
5.4
通讯作者：
Desprès, P
中科院分区：
文献类型：
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作者：
Courageot, MP;Frenkiel, MP;Desprès, P
我们报道内质网α -葡萄糖苷酶抑制剂对登革热（DEN）病毒具有抗病毒作用。我们发现葡萄糖苷酶抑制强烈影响包膜糖蛋白prM（M[膜蛋白]的细胞内糖基化前体）和E（包膜蛋白）的有效折叠途径：带有未加工的N -连接寡糖的prM的正确折叠效率低下，这导致prME异二聚体形成延迟。不完全折叠的prM和E之间形成的复合物似乎不稳定，从而导致非生产性途径。因此，抑制α -葡萄糖苷酶介导的N -连接寡糖修剪可能通过影响包膜糖蛋白加工的早期阶段来阻止登革热病毒的组装。
We report that endoplasmic reticulum or-glucosidase inhibitors have antiviral effects on dengue (DEN) virus. We found that glucosidase inhibition strongly affects productive folding pathways of the envelope glycoproteins prM (the intracellular glycosylated precursor of M [membrane protein]) and E (envelope protein): the proper folding of prM bearing unprocessed N-linked oligosaccharide is inefficient, and this causes delayed formation of prME heterodimer. The complexes formed between incompletely folded prM and E appear to be unstable, leading to a nonproductive pathway. Inhibition of or-glucosidase-mediated N-linked oligosaccharide trimming may thus prevent the assembly of DEN virus by affecting the early stages of envelope glycoprotein processing.